Polymer carrier and method for carrying out scientific, analytical and diagnostic examinations

• US 4,219,334 A
• Filed: 03/03/1978
• Issued: 08/26/1980
• Est. Priority Date: 03/05/1977
• Status: Expired due to Term

• Current Assignee: Merck Patent GmbH (Merck & Co., Inc.)
• Original Assignee: Battelle-Institut EV
• Inventors: Schluter, Gert, Schuster, Wilhelm
• Primary Examiner(s): Marantz, Sidney
• Application Number:
  US05/883,190
• Time in Patent Office: 907 Days
• Field of Search: 23/230 B, 23/253 TP, 252/408, 422/56, 424/3, 424/8
• US Class Current: 436/166
• CPC Class Codes: G01N 33/521 Single-layer analytical elements
• CPC Class Codes: G01N 33/545 Synthetic resin
• CPC Class Codes: Y10S 436/903 Diazo reactions
• CPC Class Codes: Y10T 436/146666 Bile pigment

Abstract

A polymer carrier for scientific, analytical or diagnostic examinations. The carrier consists of a soluble polymer in which the materials to be examined and/or required for examinations are incorporated and which is fully dissolved for complete release of said materials.

Claims

1. A process for preparing a product for scientific, analytical or diagnostic examination which comprises the steps:

(a) admixing a material to be examined and/or a substance required for the examination with a solution comprised of a soluble carrier and a solvent for the carrier, and

(b) evaporating off the solvent, the material and/or substance being uniformly dispersed in the soluble carrier, and said product being formed,

said product comprising the material and/or substance and the soluble carrier, the soluble carrier consisting of a soluble polymer, the material to be examined and/or the substance required for the examination being uniformly dispersed and hermetically sealed without exposure to air in the soluble carrier being capable of fully dissolving in a solvent for the carrier, whereby there is a complete release of the material and/or substance.

2. The product prepared by the process of claim 1.

3. A product as claimed in claim 2 wherein the soluble polymer is soluble in water and/or ethanol.

4. A product as claimed in claim 2 wherein the soluble polymer is selected from the group polyvinyl alcohol and polyvinyl pyrrolidone.

5. A product as claimed in claim 2 wherein the polymer is soluble in water and/or ethanol.

6. A product as claimed in claim 2 wherein the substance is a chemical or biological, reagent, a stain, an immunofluorescent reagent, an enzyme substrate/enzyme/immuno reagent mixture and/or a buffer salt.

7. A product as claimed in claim 6 wherein the material is microorganisms or cells.

8. A product as claimed in claim 7 wherein the carrier is in the form of a film or foil.

9. A product as claimed in claim 8 wherein the carrier is in the form of at least two sandwiched layers.

10. A product as claimed in claim 9 wherein the material is cells.

11. A product as claimed in claim 2 wherein the substance is a chemical or biological reagent, a stain, an immunofluorescent reagent, an enzyme substrate, an enzyme, an enzyme substrate/enzyme/immuno reagent mixture and/or a buffer salt.

12. A product as claimed in claim 2 wherein the material is microorganisms or cells.

13. A product as claimed in claim 2 wherein the carrier is in the form of a film or foil.

14. A product as claimed in claim 2 wherein the carrier is in the form of at least two sandwiched layers.

15. A product as claimed in claim 14 wherein the material is cells.

16. A product as claimed in claim 2 wherein the evaporation is achieved at room temperature.

17. A as claimed in claim 2 where the solvent used in step (a) is water and/or ethanol.

18. A method for scientific, analytical or diagnostic examinations which comprises contacting the product of claim 2 with a solvent, whereby the polymer carrier in such product is dissolved in such solvent fully releasing the homogeneously dispersed material and/or substance into such solvent immediately before examination, a solution being formed, and using such solution for a scientific analytical or diagnostic examination.

19. A method as claimed in claim 18 wherein the carrier is dissolved in water and/or ethanol.

20. A method as claimed in claim 18 wherein the polymer carrier is soluble in the body fluids to be examined and the substance required for the examination are reactive with the body fluids and are incorporated in the carrier in accurately dosed quantities, and the carrier is dissolved in a suitable amount of sample body fluid and the reaction products are determined.

21. A method as claimed in claim 18, wherein the substance required for the examination has been incorporated into the soluble polymer carrier, the product is wetted on one side with a suitable solvent, the wetted side of the product is brought into contact with a material to be examined, and the product is subsequently fully dissolved in a solvent immediately before examination.

22. A method as claimed in claim 21 wherein the side of the product is wetted with water and/or ethanol.

23. A method as claimd in claim 21 wherein the product is subsequently fully dissolved in water and/or ethanol.