Sampling and determination of diffusible chemical substances

• US 4,221,567 A
• Filed: 12/23/1977
• Issued: 09/09/1980
• Est. Priority Date: 12/23/1977
• Status: Expired due to Term

• Current Assignee: Intermountain Health Care Incorporated (IHC Hospitals, Inc.)
• Original Assignee: Intermountain Health Care Incorporated (IHC Hospitals, Inc.)
• Inventors: Wallace, William D., Clark, Justin S., Farr, Frederick L.
• Primary Examiner(s): Michell, Robert W.
• Assistant Examiner(s): Jaworski, Francis J.
• Application Number:
  US05/863,802
• Time in Patent Office: 991 Days
• Field of Search: 128/2 E, 128/2.1 E, 128/2 L, 128/2 G, 128/2.07, 128/635, 128/642, 204/195 B, 204/195 T, 23/232 R, 23/232 E, 23/230 B, 23/928, 73/16, 73/19, 73/23, 422/83, 422/98, 422/108, 422/110, 422/68
• US Class Current: 436/68
• CPC Class Codes: A61B 5/145 Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissuemeasuring of blood pressure or blood flow A61B5/02; non-radiation detecting or locating of foreign bodies in blood A61B5/06
• CPC Class Codes: A61B 5/14528 invasively
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/1495 Calibrating or testing of in-vivo probes
• CPC Class Codes: G01N 33/4925 measuring blood gas content, e.g. O2, CO2 , HCO3

Abstract

Method and system for sampling and determining chemical substances such as blood gases, in a fluid matrix, such as blood, by bringing the chemical substances into equilibrium with a liquid in a fiber probe, passing the equilibrated liquid to a sensor adjacent the equilibrium region and on into a hollow fiber line enclosed in a calibration chamber. Calibration of the sensors is accomplished by reversing the flow of liquid from the hollow fiber line to the sensors. In the system chemical substances for sensor calibration are provided by an electric proportioner whose output is continuously controlled by the output of the sensors such that the substances proportioned into a fluid in the calibration chamber surrounding the hollow fiber line are substantially the same as the substances within the matrix. The concentration of substances is determined by the output of the sensors and the amount of substances fed from the proportioner to the fluid surrounding the hollow fiber line. When the substances being measured are in equilibrium with both the matrix and fluid surrounding the hollow fiber line, a null response is obtained at the sensors.

Claims

1. A method for determining the concentration of diffusible gaseous substances in a fluid matrix comprising:

(a) introducing a probe into said fluid matrix, said probe containing an outer membrane permeable to the substances being determined and consisting of interconnected outer and inner chambers, the walls of said inner chamber being less permeable to the substances being determined than said membrane,

(b) introducing an equilibration liquid of limited solubility to the substances being determined into said outer chamber in contact with said membrane,

(c) allowing said gaseous substances to diffuse across said membrane and equilibrate with said equilibration liquid,

(d) withdrawing said equilibration liquid equilibrated with said gaseous substances from said outer chamber through said inner chamber and introducing it to a sensor chamber containing sensors which are specific for the substances being determined, the electrical output of a specific sensor being representative of the concentration of a particular gaseous substance in said fluid matrix,

(e) relaying the electrical output obtained at each sensor to controller means wherein it is stored.

(f) utilizing the stored signals in said controller means to actuate a chemical feeding means such that the chemical feeding means introduces a known concentration of gases being determined into a calibration chamber such that the concentration of each gas in the calibration chamber is known

(g) passing said equilibration liquid from said sensor chamber to a hollow fiber means within the calibration chamber wherein the equilibration liquid equilibrates with the known concentrations of gaseous substances in said calibration chamber

(h) passing the equilibration liquid from the hollow fiber means within the calibration chamber by reverse flow back to the sensor chamber whereby said sensors are calibrated with the equilibration liquid equilibrated with known concentrations of the gaseous substances being determined and the electrical output of each sensor calibrated is relayed to said controller means and stored.

2. A method according to claim 1 wherein the concentration of each gas in the calibration chamber is the same as that previously determined to be in the fluid matrix of the sensors and further comprising,

(a) passing the equilibration liquid used to calibrate the sensors from the sensor chamber to the outer chamber of the probe and,

(b) repeating steps (b) through (h) of claim 1 whereby the known concentrations of gaseous chemical substances in the equilibration means are altered in stages by the chemical feeding means until the stored sensor responses to equilibrated liquid from the inner chamber of the probe and from the hollow fiber means within the calibration chamber are the same.

3. A method according to claim 2 wherein the equilibration liquid serves as a common electrolyte for said sensors.

4. A method according to claim 3 wherein the body liquid is blood.

5. A method according to claim 4 wherein said equilibration liquid is a saline-bicarbonate solution.

6. A method according to claim 4 wherein the probe is inserted into an artery.

7. A method according to claim 6 wherein the gases being determined are oxygen and carbon dioxide.

8. A method according to claim 3 wherein the chemical feeding means is a gas proportioner.

9. A system for determining the concentration of diffusible gaseous substances in a fluid matrix comprising:

(a) a probe containing consentric outer membrane permeable to the substances being determined and consisting of an interconnected outer and inner chambers, the walls of said inner chamber being less permeable to the substances being determined than said membrane, the outer chamber containing liquid inlet means and the inner chamber containing liquid outlet means,

(b) a sensing chamber in liquid communication with said inner and outer probe chambers containing sensors electrically sensitive to the gaseous substances in proportion to the concentrations thereof,

(c) controller means adapted to receive, store and compare signals received from said sensors and issue commands responsive thereto,

(d) hollow fiber means in liquid communication with the sensing chamber and surrounded by a calibration chamber,

(e) chemical feeding means in electrical communication with said controller means adapted to feed variable known concentrations of the gaseous substances being determined into the calibration chamber as commanded by said controller means in response to the signals received from said sensors, and

(f) bidirectional means interconnecting the liquid inlet to the probe to the hollow fiber means and adapted to regulate the direction and flow rate of liquid through the system.

10. A system according to claim 9 wherein the outer membrane of the probe is a silicone polymer or copolymer.

11. A system according to claim 10 wherein the chemical feeding means is a gas proportioner.

12. A system according to claim 11 wherein the sensors are electrodes specific to the determination of blood gases.

13. A system according to claim 12 wherein the blood gases are carbon dioxide and oxygen.

14. A system according to claim 13 wherein the probe is adapted to be inserted through a catheter and into an artery.