Process for mounting palynological specimens

• US 4,221,823 A
• Filed: 04/21/1978
• Issued: 09/09/1980
• Est. Priority Date: 04/21/1978
• Status: Expired due to Term

• Current Assignee: Phillips Petroleum Company (ConocoPhillips)
• Original Assignee: Phillips Petroleum Company (ConocoPhillips)
• Inventors: Shell, Francis J., Pearson, Michael B., Meyer, Sharon A.
• Primary Examiner(s): Hayes, Lorenzo B.
• Application Number:
  US05/898,552
• Time in Patent Office: 872 Days
• Field of Search: 427/2, 427/4, 424/3, 156/57, 350/92
• US Class Current: 427/4
• CPC Class Codes: G01N 1/28 Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Qmounting specimens on microscopic slides G02B21/34; means for supporting the objects or the materials to be analysed in electron microscopes H01J37/20 ; laboratory gas handling apparatus B01L5/00

Abstract

A process for mounting palynological specimen on a support employing a solution comprising water and at least one substance selected from the group consisting of water-soluble salts of carboxyalkyl hydroxyalkyl cellulose and water-soluble salts of carboxyalkyl cellulose.

Claims

1. A process comprising:

forming a solution comprising water and at least one water-soluble salt selected from the group consisting of carboxymethyl cellulose, carboxyethyl cellulose, carboxypropyl cellulose, carboxymethyl hydroxyethyl cellulose, carboxyethyl hydroxyethyl cellulose, and carboxymethyl hydroxymethyl cellulose wherein the amount of the water-soluble salt is within the range of about 0.3 to about 1.5 weight percent based on the weight of the solution,

placing at least a portion of said solution on a support along with a suitable amount of a specimen.

2. A process according to claim 1 further comprising evaporating the water from said solution to produce a film which secures said specimen to said support.

3. A process according to claim 2 wherein said water is evaporated by heating said support after said solution and said specimen are placed thereon.

4. A process according to claim 3 wherein said support is at least one light-transmitting plate.

5. A process according to claim 4 wherein said support is a cover glass.

6. A process according to claim 1 wherein said water is distilled water.

7. A process according to claim 1 wherein the amount of watersoluble salt is within the range of about 0.4 to about 0.8 weight percent based on the weight of the solution.

8. A process according to claim 1 wherein the amount of watersoluble salt is within the range of about 0.4 to about 0.6 weight percent based on the weight of the solution.

9. A process according to claim 1 wherein the water-soluble salts are selected from the group consisting of metal salts of Group IA metals of the Periodic Table of the Elements and ammonium salts.

10. A process according to claim 9 wherein the Group IA metals are selected from the group consisting of lithium, sodium, and potassium.

11. A process according to claim 10 wherein the water-soluble salt is sodium carboxymethyl cellulose.

12. A process according to claim 11 wherein said sodium carboxymethyl cellulose has a degree of substitution in the range of 0.7 to 1.2.

13. A process according to claim 12 wherein said sodium carboxymethyl cellulose has a degree of substitution of about 0.9.

14. A process according to claim 5 wherein said support is a cover glass attached to a glass slide wherein the specimen is positioned between said cover glass and said glass slide.

15. A process according to claim 1 wherein said solution is formed consisting essentially of said water and said at least one water-soluble salt.

16. A process according to claim 1 wherein said specimen is selected from the group consisting of microorganisms and microfossils.

17. A process according to claim 16 wherein said microfossils are acid-insoluble.

18. A process according to claim 1 wherein said water is distilled water, said water-soluble salt is sodium carboxymethyl cellulose having a degree of substitution of about 0.9 wherein the amount of said sodium carboxymethyl cellulose is within the range of about 0.4 to about 0.6 weight percent based on the weight of the solution, said support is a cover glass and said specimen is an acid-insoluble microfossil.