Method for determining endotoxin concentration
First Claim
1. In an optical method for determining endotoxin wherein a sample thought to contain endotoxin is contacted with an endotoxin-coagulable protein from Limulus amebocytes to form a reaction product which contains coagulated protein, and then the light scattering or absorbence of a suspension of such reaction product determined, the improvement comprising contacting the reaction product with a sufficient amount of monofunctional ionic detergent to dissolve said coagulated protein, with the proviso that the reaction product is not contacted with a suspending agent.
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Abstract
Improvements in the sensitivity and reproducibility of nephelometric endotoxin determinations using endotoxin-activated enzyme from Limulus amebocyte lysate are achieved by contacting endotoxin-coagulated lysate with an ionic surface active suspending agent, generally a mixture of detergent and suspending agent. Instrumentation improvements further enhance results.
29 Citations
15 Claims
- 1. In an optical method for determining endotoxin wherein a sample thought to contain endotoxin is contacted with an endotoxin-coagulable protein from Limulus amebocytes to form a reaction product which contains coagulated protein, and then the light scattering or absorbence of a suspension of such reaction product determined, the improvement comprising contacting the reaction product with a sufficient amount of monofunctional ionic detergent to dissolve said coagulated protein, with the proviso that the reaction product is not contacted with a suspending agent.
Specification