Objective determination of the rate of oxygen utilization in peripheral tissue

• US 4,222,389 A
• Filed: 10/18/1977
• Issued: 09/16/1980
• Est. Priority Date: 10/18/1977
• Status: Expired due to Term

• Current Assignee: Institute of Applied Biology Special Cancer Research Project
• Original Assignee: Institute of Applied Biology Special Cancer Research Project
• Inventors: Rubens, Harry E.
• Primary Examiner(s): Howell, Kyle L.
• Application Number:
  US05/843,270
• Time in Patent Office: 1,064 Days
• Field of Search: 128/2 L, 128/2 A, 128/2 R, 128/2 G, 128/2.07, 128/633, 128/637, 128/666, 356/39-41
• US Class Current: 600/328
• CPC Class Codes: A61B 10/00 Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis vaccination prophylaxis, vaccination therapy A61B17/20; Sex determination; Ovulation-period determination menstruation tables G06C3/00; Throat striking implements
• CPC Class Codes: A61B 90/06 Measuring instruments not otherwise provided for

Abstract

A non-invasive method of objectively determining, in vivo, the rate of oxygen utilization in peripheral tissue by spectral measuring means, which involves isolating a segment of blood in the tissue with a clamp or tourniquet, and obtaining rapid, successive spectral curves of the oxyhemoglobin in the occluded area, to and from selected wavelengths of light, and measuring the time-space relationship between a rapidly moving portion of the spectral curve and a slower moving adjacent portion of the same curve, from the moment of occlusion, to establish a rate of reduction of the oxyhemoglobin in the tissue which is directly correlated to the rate of oxygen utilization.

Claims

1. A non-invasive, spectrometric method of objectively determining in vivo, the rate of oxygen utilization in peripheral tissue, which comprises isolating a segment of blood in the tissue of the skin, and simultaneously and rapidly obtaining from the moment of blood isolation, the changing light coefficients of absorption for oxyhemoglobin in the isolated blood, at two adjacent wavelengths in which the coefficients of absorption of one wavelength, has a faster rate of change than the other, and determining the rate of change of one light coefficient with respect to the other.

2. The method of claim 1, in which the selected wavelengths of oxyhemoglobin are taken from adjacent wavelengths at the 245, 280, 350, 417, 548 and 578 nm.

3. The method of claim 1, in which the adjacent wavelengths are about 565 and 578 nm.

4. The method of claim 1, in which the rate of change of one light coefficient with respect to the other is measured by the time required from the moment of blood isolation, for one coefficient of absorption to equal that of the other.

5. The method of claim 1, in which the rate of change of one light coefficient with respect to the other is measured by the time required from the moment of blood isolation, for the coefficient of absorption at 578 nm, to equal the coefficient of absorption at about 565 nm.