Method and apparatus for chemical analysis

• US 4,224,032 A
• Filed: 12/05/1977
• Issued: 09/23/1980
• Est. Priority Date: 12/17/1976
• Status: Expired due to Term

• Current Assignee: Clinical Diagnostic Systems Inc.
• Original Assignee: Eastman Kodak Company
• Inventors: Meyer, Robert J., Muka, Edward, Glover, Clyde P., Ferris, James E.
• Primary Examiner(s): Serwin, R. E.
• Application Number:
  US05/857,344
• Time in Patent Office: 1,023 Days
• Field of Search: 23/230 R, 23/230 B, 422/64-66, 195/127, 424/3, 353/113, 119/35, 119/39, 119/43, 435/809
• US Class Current: 436/46
• CPC Class Codes: G01N 2035/00039 Transport arrangements specific to flat sample substrates, e.g. pusher blade
• CPC Class Codes: G01N 2035/00049 for loading/unloading a carousel
• CPC Class Codes: G01N 2035/00079 Evaporation covers for slides
• CPC Class Codes: G01N 2035/00089 Magazines
• CPC Class Codes: G01N 2035/00356 Holding samples at elevated temperature (incubation)
• CPC Class Codes: G01N 2035/00386 using fluid heat transfer medium
• CPC Class Codes: G01N 35/00029 provided with flat sample substrates, e.g. slides G01N35/028 takes precedence
• CPC Class Codes: Y10S 435/809 Incubators or racks or holders for culture plates or containers
• CPC Class Codes: Y10T 436/112499 with sample on test slide
• CPC Class Codes: Y10T 436/144444 Glucose
• CPC Class Codes: Y10T 436/171538 Urea or blood urea nitrogen

Abstract

A method and apparatus are disclosed for performing chemical analysis on selected fluids. The apparatus comprises a turntable which carries a plurality of cartridges containing test slides. A transfer mechanism is adapted to receive a slide from a selected cartridge, transport the slide to a metering device where a precise amount of fluid is deposited thereon, and then deliver the slide to a conveyor which moves the slide through an incubator. A radiometric reading of the slide is taken while it is in the incubator.

Claims

1. A method of performing chemical analysis of a fluid comprising the steps of:

(a) removing a slide from a stack of slides, each of said slides including means to effect the analysis of a fluid sample;

(b) transporting said slide to a metering position;

(c) metering a predetermined quantity of fluid on said slide;

(d) transporting said slide to incubator means, said incubator means having a plurality of conveyor means;

(e) depositing said slide in a selected one of said conveyor means;

(f) conveying said slide to a position to cooperate with an analysis means after an appropriate period of incubation; and

(g) sensing a characteristic of said slide resulting from the fluid deposited thereon.

2. A method, as defined in claim 1, wherein said fluid is stored in a container and the fluid is metered from said container.

3. A method, as defined in claim 2, wherein said sensing of the slide is performed while said slide is in said conveyor means.

4. In an automatic chemical analyzer comprising sample supply means for receiving a plurality of containers of fluid sample, reagent supply means for receiving a stack of slides each of which contains a reagent, metering means adapted to dispense a predetermined quantity of fluid sample onto a slide, and incubator means, the improvement wherein:

(a) conveyor means is provided in said incubator means, said conveyor means having a plurality of members for receiving slides bearing fluid sample and means for advancing the slides through said incubator means;

(b) slide transporting means is provided for removing a slide from said stack, transporting said slide to a metering position where said quantity of fluid sample is deposited on the slide by said metering means, and delivering the slide to a selected one of said members; and

(c) analysis means is operatively associated with said conveyor means.

5. An analyzer, as defined in claim 4, wherein said slide transporting means includes ejector means for removing a slide from said reagent supply means and transfer means adapted to receive a slide from said ejector means.

6. An analyzer, as defined in claim 5, wherein said transfer means includes a transfer arm having gripping means adapted to receive and hold a slide, said arm is mounted for rotation in either of two directions, and a pusher member is adapted to move a slide out of said gripping means.

7. An analyzer, as defined in claim 6, wherein said metering means is adapted to form a pendant drop, and said metering means and said transfer arm are movable relative to each other to deposit said drop on a slide in said arm.

8. An analyzer, as defined in claim 7, wherein said incubator devices have different operating characteristics whereby different analyses are carried out in the respective devices.

17. An analyzer, as defined in claim 8, wherein said incubator means includes a housing, said conveyor means comprises a rotor in said housing, and said slide holding members are spaced around the periphery of the rotor.

18. An analyzer, as defined in claim 17, wherein said analysis means is at least partially located within said housing and is adapted to measure radiation reflected from a slide held in said rotor.

9. A chemical analyzer for measuring a characteristic of a fluid, said analyzer comprising in combination:

slide supply means for receiving at least one stack of slides, each slide including means to effect the analysis of a fluid;

sample supply means for receiving a plurality of containers of fluids to be analyzed;

metering means for depositing a predetermined quantity of fluid onto a slide supported in a metering position;

incubator means for providing a controlled environment for slides bearing said fluid, said incubator means having a loading station for receiving slides;

conveyor means operative in said incubator means, said conveyor means comprising a plurality of slide holding members each of which is adapted to receive and releasably hold a slide, said conveyor means further comprising means for advancing said slide holding members past said station;

analysis means operative after a slide has been in the incubator means a predetermined time for sensing a characteristic of said slide resulting from the fluid deposited thereon; and

slide transporting means for removing a slide from said stack, moving the slide to said metering position, and delivering the slide to a selected slide holding member in said conveyor means.

10. A chemical analyzer, as defined in claim 9, wherein said slide contains a reagent which is adapted to react with a component of said fluid to effect a change which can be radiometrically detected.

11. An automatic chemical analyzer, as defined in claim 9, wherein said slide is capable of potentiometrically designating the activity of ions in said fluid.

12. An analyzer, as defined in claim 9, wherein said slide transporting means includes ejector means for removing a slide from said slide supply means and transfer means adapted to receive a slide from said ejector means.

13. An analyzer, as defined in claim 12, wherein said slide supply means includes a table mounted for rotation and adapted to receive and support a plurality of slide stacks, and said ejector means includes a pusher element adapted to move a slide from one of said stacks into gripping means on said transfer means.

14. An analyzer, as defined in claim 12, wherein said transfer means includes a rotatably mounted transfer arm for supporting a slide and a pusher member for moving the slide from the arm into said conveyor means.

15. An analyzer, as defined in claim 14, wherein said metering means is adapted to form a pendant drop, and said transfer arm and said metering means are movable relative to each other to deposit said drop on the slide supported by said arm.

16. An analyzer, as defined in claim 9, wherein said sample supply means includes a carrier adapted to receive said containers, and said carrier being adapted to successively position said containers adjacent said metering means.

19. An analyzer, as defined in claim 9, wherein said incubator means includes a pair of housings, said conveyor means includes a conveyor in each of said housings, and said slide transporting means is adapted to selectively deliver a slide to either of said conveyors.

20. An analyzer, as defined in claim 9, wherein said slide transporting means comprises transfer means, and said transfer means and said slide supply means are rotatable in either of two directions.

21. An analyzer, as defined in claim 9, wherein each of said containers comprises a metering tip and a cap covering said tip, and means are provided for automatically removing said cap at the metering position.

22. An analyzer, as defined in claim 9, wherein said slide supply means comprises a supply table having a plurality of slide stacks, said sample supply means includes a rotatively mounted sample tray and said containers are spaced around the periphery thereof, and said slide transporting means comprises transfer means mounted for rotation relative to said table and said tray.

23. An analyzer, as defined in claim 9, wherein programmed means are provided for automatically controlling sequential operation of said means.

24. An analyzer for measuring a characteristic of a fluid sample wherein the sample is deposited on a test slide which is analyzed after an appropriate period of time under controlled conditions, said analyzer comprising:

slide supply means for receiving a plurality of stacks of slides, one of said stacks including slides to effect an analysis of a first type and another of said stacks including slides to effect an analysis of a second type;

sample supply means for receiving a plurality of discrete samples;

metering means adapted to deposit a predetermined quantity of fluid from a selected sample onto a slide in a metering position;

incubator means including a plurality of temperature controlled chambers and conveyor means for advancing slides within the chambers, on of said chambers being adapted to accept slides of said first type and another of said chambers being adapted to accept slides of the second type;

analysis means adapted to cooperate with each of said chambers, said analysis means being operative after a slide has been in a chamber for a predetermined time to sense a characteristic of the slide resulting from the fluid deposited thereon; and

slide transport means for removing a slide from a stack, moving the slide to said metering position and delivering the slide to a selected one of said chambers.

26. An analyzer, as defined in claim 24, wherein;

said slide transport means includes a transfer means mounted for rotation through a circular path, and said slide supply means, sample supply means, metering means, and incubator means are arranged at spaced locations along said path.

27. An analyzer, as defined in claim 24, wherein said slide transport means, said conveyor means, said sample supply means, and said slide supply means are mounted for rotation.

25. An analyzer for measuring a characteristic of a fluid sample wherein a sample is deposited on a test slide which is analyzed after an appropriate period of incubation, said analyzer comprising:

sample supply means for receiving fluid samples to be analyzed;

slide supply means for receiving a plurality of types of test slides and for delivering a selected type of test slide to a feed position;

metering means for depositing a predetermined quantity of sample on a test slide;

incubator means including a plurality of separate devices arranged to operate on individual slides;

analysis means for sensing a characteristic of a slide resulting from the fluid sample deposited thereon, said analysis means being adapted to cooperate with each of said devices;

means for transporting a slide from said supply means to said metering means and from said metering means to a selected one of said devices; and

means for controlling the operation of said previously recited means in a preselected sequence to provide for the continuous analysis of fluid samples.

28. Slide transporting means for use in a chemical analyzer of the type in which a fluid is metered onto a test slide which is analyzed after an appropriate period of incubation, said analyzer having a plurality of elements arranged along and outside of a circular path, said elements comprising slide supply means, sample supply means, metering means, and incubator means, said slide transporting means comprising:

transfer means for receiving a slide from said slide supply means, moving the slide to said metering means, and delivering the slide to said incubator means, said transfer means being movable through said circular path; and

control means for moving said transfer means in timed relation to said analyzer elements.