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Apparatus for measuring cytological properties

  • US 4,225,229 A
  • Filed: 03/06/1978
  • Issued: 09/30/1980
  • Est. Priority Date: 03/04/1977
  • Status: Expired due to Term
First Claim
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1. A flow chamber for use in an optical system for detecting characteristics of cells suspended in a liquid, particularly for observation of the fluorescence characteristics of the cells upon excitation thereof by light sources having different wavelengths, comprisinga body having a transparent wall portion;

  • andmeans in said body for defining a flow passage through which the liquid and cells can pass, said flow passage having first and second passage portions intersecting at said wall portion and forming an angle therebetween of significantly less than 180°

    ;

    said means including an edge defining a knee between said first and second portions, said edge being spaced from said transparent wall portion and lying in a plane extending across said first and second passage portions, so that cells in liquid flowing through said passages pass through said plane in one direction toward said wall portion and through said plane in the other direction away from said wall portion on opposite sides of said edge whereby the fluorescence characteristics of the cells can be stimulated and observed through said wall portion as they pass in said respective directions through said plane.

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