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Process for separation and collection of viable female and male spermatozoa

  • US 4,225,405 A
  • Filed: 08/16/1978
  • Issued: 09/30/1980
  • Est. Priority Date: 08/16/1978
  • Status: Expired due to Term
First Claim
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1. A process for separating and collecting viable female spermatozoa (XX chromosome) and male spermatozoa (XY chromosome) in a semen sample, which process comprises subjecting the semen sample to negative pressure to cause the male spermatozoa to move to the top of the sample, wherein the semen sample is brought into contact with a semen extender material, the semen extender material being disposed in surmounting relation to the sample, the male spermatozoa migrating into the semen extender material on subjection of the sample and the semen extender material to negative pressure.

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