Process for separation and collection of viable female and male spermatozoa
First Claim
1. A process for separating and collecting viable female spermatozoa (XX chromosome) and male spermatozoa (XY chromosome) in a semen sample, which process comprises subjecting the semen sample to negative pressure to cause the male spermatozoa to move to the top of the sample, wherein the semen sample is brought into contact with a semen extender material, the semen extender material being disposed in surmounting relation to the sample, the male spermatozoa migrating into the semen extender material on subjection of the sample and the semen extender material to negative pressure.
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Abstract
A process is disclosed for separating and collecting viable female spermatozoa (XX chromosome) and male spermatozoa (XY chromosome). The process utilizes apparatus comprising two sterilized columns of glass, plastic or other suitable material, a ball valve, a vacuum pump, a mercury manometer, and connecting tubes of glass, plastic or other suitable materials. The system is assembled with rubber percussion gaskets or other suitable connecting materials, to prevent the introduction of extraneous air into the closed system, and a burp bottle to preclude unwanted introduction of fluids into parts of the system under vacuum. The column containing the semen sample may be of variable volume, to accommodate semen samples of varying volume and concentration.
The process may be modified by introducing into the system a direct and continuous electrical current flow, or by creating within the separating columns an electrophoretic field. The process may be further modified into a continuous-flow system by adding another mercury manometer, a Cartesian Diver regulator, a second burp bottle, and an over-flow collecting bottle connected to the two main columns.
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Citations
8 Claims
- 1. A process for separating and collecting viable female spermatozoa (XX chromosome) and male spermatozoa (XY chromosome) in a semen sample, which process comprises subjecting the semen sample to negative pressure to cause the male spermatozoa to move to the top of the sample, wherein the semen sample is brought into contact with a semen extender material, the semen extender material being disposed in surmounting relation to the sample, the male spermatozoa migrating into the semen extender material on subjection of the sample and the semen extender material to negative pressure.
Specification