Process for the dehydration of a colloidal dispersion of lipsomes

• US 4,229,360 A
• Filed: 08/04/1978
• Issued: 10/21/1980
• Est. Priority Date: 08/05/1977
• Status: Expired due to Term

• Current Assignee: The Liposome Co., Inc. (Perrigo Company PLC)
• Original Assignee: Battelle Memorial Institute
• Inventors: Lamy, Bernard, Schneider, Michel
• Primary Examiner(s): Niebling, John F.
• Application Number:
  US05/931,243
• Time in Patent Office: 809 Days
• Field of Search: 260/403, 426/98, 426/99, 426/385, 426/663, 252/316, 424/36, 424/178, 424/199
• US Class Current: 264/4.6
• CPC Class Codes: A61K 9/127 Liposomes

Abstract

The invention concerns a process for the dehydration of a colloidal dispersion of liposomes in an aqueous liquid medium, this process being aimed at extending the conservation of the liposomes and to enable their efficient use at a later date.

According to this process, there is prepared a mixture of a hydrophilic compound and the liposome dispersion. This mixture is then subjected to a dehydration operation leading to the formation of liposomes in the form of a stable powder which can be stored for a long period and from which a dispersion of liposomes can be reconstituted.

Claims

1. A process for the dehydration of a colloidal dispersion of liposomes in an aqueous liquid medium, which comprises mixing a hydrophilic compound with the liposome dispersion and dehydrating the mixture to form a stable liposome containing powder which can be stored and reconstituted in an aqueous medium as a liposome dispersion.

2. The process of claim 1, in which the mixture is dehydrated by first freezing said mixture of the liposome dispersion and the hydrophilic compound and, thereafter, removing water from said frozen mixture by reduced pressure evaporation to obtain said powder of liposomes.

3. The process of claims 1 or 2, wherein the liposomes consist essentially of vesicles of a liquid encapsulated in a film of a lipid and said mixture includes, by weight, at least as much of the hydrophilic compound as of the lipid used to form the liposomes.

4. The process of claim 3 wherein the weight ratio of the hydrophilic compound to the lipid is from 1 to 1 to 4 to 1.

5. The process of claim 3, wherein said hydrophilic compound is selected from the group comprising dextran, gum arabic, polyvinyl alcohol, polyvinyl pyrrolidone and ox-albumin.