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Breast cyst fluid protein assay

  • US 4,229,426 A
  • Filed: 02/22/1978
  • Issued: 10/21/1980
  • Est. Priority Date: 02/22/1978
  • Status: Expired due to Term
First Claim
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1. A method for determing the concentration of the glycoprotein gross cystic disease fluid protein-15 (GCDFP-15) in a biological fluid sample which method comprises conducting an immunoassay for said glycoprotein in said sample utilizing as reagents a GCDFP-15 selective antibody and GCDFP-15 labelled with a unique and detectable label to produce an antigen-antibody reaction product, separating said product from the said reagents and determining the concentration of GCDFP-15 in said sample by detecting the quantitative value of said label in either said reaction product or said reagents and comparing said label value to a standard curve wherein said GCDFP-15 is further characterized as follows:

  • (a) a glycoprotein having a calculated monomer size of about 15,000 daltons as determined by sodium dodecyl sulfate acrylamide gel analysis; and

    (b) immunologically not identical to any components of plasma as determined by Ouchterlony analysis; and

    (c) immunological cross identity with a component of human milk and human saliva.

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