Reagent for use in immunochemical assay methods

• US 4,232,119 A
• Filed: 03/02/1978
• Issued: 11/04/1980
• Est. Priority Date: 03/04/1977
• Status: Expired due to Term

• Current Assignee: Pharmacia Diagnostics AB (Thermo Fisher Scientific Incorporated)
• Original Assignee: Pharmacia Diagnostics AB (Thermo Fisher Scientific Incorporated)
• Inventors: Axen, Rolf E. A. V., Carlsson, Jan P. E., Drevin, Hakan N. Y.
• Primary Examiner(s): Shapiro, Lionel M.
• Application Number:
  US05/882,545
• Time in Patent Office: 978 Days
• Field of Search: 195/63, 195/68, 195/103.5 A, 424/1, 424/12, 260/112 R, 260/112 B, 23/230 B, 435/7
• US Class Current: 530/391.5
• CPC Class Codes: A61K 39/44 Antibodies bound to carriers
• CPC Class Codes: G01N 33/532 Production of labelled immunochemicals
• CPC Class Codes: Y10S 435/964 including enzyme-ligand conjugate production, e.g. reducing rate of nonproductive linkage

Abstract

A reagent for use in immunochemical assay methods carried out in the presence of an aqueous liquid, which reagent comprises a conjugate of one or more molecules of immunoglobulin and one or more units of an analytically indicatable group which molecules and units are bound together via bridges containing the group, --S--S--, said conjugate being soluble in said aqueous liquid.

Claims

1. A reagent for use in immunochemical assay methods carried out in the presence of an aqueous liquid, wherein said reagent comprises a conjugate of one or more molecules of immunoglobulin and one or more units of an analytically indicatable group, which molecules and units are bound together via bridges containing the group --S--S--, said conjugate being soluble in said aqueous liquid.

2. A reagent according to claim 1, characterized in that the analytically indicatable group is an enzymatically active, radioactive or fluorescent group.

3. A reagent according to claim 1, characterized in that said immunoglobulin is an antibody directed against an antigen or hapten.

4. A reagent according to claims 1, 2 or 3 characterized in that the molecules of immunoglobulin and the units of the analytically indicatable group are each bound to a water-soluble carrier, at least one of said immunoglobulin and said group being bound to the carrier via bridges containing the group --S--S--.

5. A reagent according to claim 2, characterized in that said immunoglobulin is an antibody directed against an antigen or hapten.