Measuring cell for micro-assays, comprising membrane electrodes

• US 4,235,687 A
• Filed: 05/16/1978
• Issued: 11/25/1980
• Est. Priority Date: 05/20/1977
• Status: Expired due to Term

• Current Assignee: Agence Nationale de Valorisation de la Recherche (Bpifrance SA /Old/)
• Original Assignee: Agence Nationale de Valorisation de la Recherche (Bpifrance SA /Old/)
• Inventors: Romette, Jean-Louis, Thomas, Daniel, Gellf, Gerard
• Primary Examiner(s): Kaplan, G. L.
• Application Number:
  US05/906,891
• Time in Patent Office: 924 Days
• Field of Search: 204/195 P, 204/1 P, 204/1 E, 204/195 B, 204/195 M, 195/127, 324/29, 324/30 R
• US Class Current: 204/403.1
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: G01N 27/40 Semi-permeable membranes or partitions
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

The measuring cell is composed of at least one cavity hollowed in a block of chemically inert material or material made chemically inert at least at the surface. The cavity defines a first cylindrical space whose bottom is, over a reduced part of its diameter, extended by a second space defining the measuring chamber proper, of conical, frustoconical or substantially hemispherical shape. The first cylindrical space is designed to receive a measuring electrode terminated by a magnetic torus and, applied, and if necessary held by means of a ferromagnetic ring, against the latter, a membrane, while inlet and outlet ducts for the liquids to be assayed are formed in the block. The cell is especially useful for micro-assays of biological fluids.

Claims

1. Measuring cell for assays utilizing membranes, said cell being essentially composed of at least one cavity hollowed out in a block of material, so as to define a first cylindrical space whose bottom is, over a reduced part of its diameter, extended by a second space defining the measuring chamber proper, of conical, frustoconical or substantially hemispherical shape, a measuring electrode terminated by a magnetic torus within said first cylindrical space, means holding the torus in position, a non-magnetic membrane of circular shape cut out to the desired size within said cavity adjacent the magnetic torus and between the end of said torus and the mating surface of the cavity, and ducts respectively for the inlet and outlet of the liquids to be assayed and/or the passage of other fluids connecting the periphery and/or the bottom of the cell with the measuring chamber, and open into the latter.

2. Measuring cell according to claim 1, wherein said ducts open into said measuring chamber substantially tangentially with respect to the surface of the membrane immobilized in said chamber.

3. Measuring cell according to claim 1, wherein the inlet and outlet ducts for the liquids to be assayed have an inclination such that they open on to the periphery of the measuring chamber.

4. Measuring cell according to claim 1, equipped with two opposite electrodes, the measuring space then being, in a cavity obtained by drilling said block of material from side-to-side, bounded by a ferromagnetic ring suitably introduced, each of the two electrodes being provided with a membrane, while the ducts respectively for the inlet and outlet of the liquid to be assayed are arranged transversally, in opposition with respect to one another, and penetrating into said ferromagnetic ring, the two electrodes provided with their magnetic tori and with their membranes being mounted directly on both sides of the ferromagnetic ring and thus defining a single measuring cavity.

5. Measuring cell according to claim 1, wherein said membrane is an enzymatic membrane.

6. Measuring cell according to claim 1, produced in a block of metal or plastic.

7. Measuring cell according to claim 6, wherein said blocks is made of a polyacrylate.

8. Measuring cell according to claim 7, wherein said polyacrylate is polymethyl methacrylate or polyethyl methacrylate.

9. Measuring cell according to claim 6, wherein said block is made of a metal alloy.

10. Measuring cell according to claim 9, wherein said block is made of stainless steel.

11. Measuring cell according to claim 1, wherein a ferromagnetic ring constitutes the means holding the torus in position, said ferromagnetic material being constituted by the cell itself or by at least the surface of the flat bottom of the cylindrical part of the cavity.

12. Measuring cell according to claim 1, comprising, in immediate extension of the cylindrical part of said cavity and at the upper end or free end of the latter, an annular excrescence of an acute angle, suitable for directly stamping out membrane discs of desired size from a larger sheet.

13. Measuring cell according to claim 1, wherein the means holding the torus in position comprises a ferromagnetic ring supported on the bottom of the cylinder within the cavity, the membrane being held between the torus and ring.

14. Measuring cell according to claim 1, equipped with two opposite electrodes, the measuring space then being, in a cavity obtained by drilling said block of material from side-to-side, bounded by a cylindrical crown, ring or bead flattened on both sides, each of the two electrodes being provided with a membrane, while the ducts respectively for the inlet and outlet of the liquids to be assayed are arranged transversally, in opposition with one another, and penetrating into said crown, ring or bead, the two electrodes provided with their magnetic tori and with their membranes being mounted directly on both sides of the crown, ring or bead and thus defining a single measuring cavity.