Pectin culture media and method

• US 4,241,186 A
• Filed: 12/18/1978
• Issued: 12/23/1980
• Est. Priority Date: 12/18/1978
• Status: Expired due to Term

• Current Assignee: Conviron Incorporated
• Original Assignee: Conviron Incorporated
• Inventors: Roth, Jonathan N.
• Primary Examiner(s): Penland, R. B.
• Application Number:
  US05/970,347
• Time in Patent Office: 736 Days
• Field of Search: 195/100, 195/99, 195/101, 195/102, 195/103, 195/127, 195/139, 536/2, 435/299, 435/240, 435/241, 435/245, 435/243, 435/810
• US Class Current: 435/243
• CPC Class Codes: C12Q 1/045 Culture media therefor
• CPC Class Codes: Y10S 435/81 Packaged device or kit

Abstract

A method for preparing a gelled biological growth medium in a culture growth container which comprises combining a liquid growth medium including low methoxyl pectin and a multivalent metal cation material in the culture growth container. Preferably, the cation material is located on a support material, such as a filter pad or paper, and the liquid growth medium including the low methoxyl pectin material is poured over the support material while in the culture growth container.

Claims

1. A method for preparing a gelled biological growth medium having a pH from about 4 to about 9 in a culture growth container which comprises the steps of:

a. providing a culture growth container,

b. preparing a liquid growth medium including between about ten and about thirty grams per liter of growth medium of a low methoxyl pectin material having less than about seven percent methoxyl content to gel the growth medium as the sole gelling agent;

c. introducing a predetermined amount of calcium ions into an absorbent support material and placing the absorbent support material into the culture growth container; and

d. combining the growth medium and the calcium ions in said culture growth container to produce gelling of the growth medium.

2. The method of claim 1 in which the low methoxyl pectin material has approximately a five percent methoxyl content.

3. A method for preparing a gelled biological growth medium in a culture growth container which comprises the steps of:

a. providing a culture growth container, said container including therein an absorbent support material containing calcium ions;

b. providing a liquid growth medium having a pH from about 4 to about 9 including between about ten and about thirty grams per liter of growth medium of a low methoxyl pectin material having less than about seven percent methoxyl content as the sole gelling agent for gelling the growth medium; and

c. adding the growth medium to the culture growth container in an amount to provide gelling of the growth medium upon contact of the calcium ions with the pectin.

4. The method of claim 3 in which the liquid growth medium includes a buffer selected from the group consisting of Na₂ HPO₄, NaH₂ PO₄, Na₃ PO₄, NaHCO₃ and Na₂ CO₃.

5. The method of claim 3 in which the calcium cations and liquid growth medium including pectin are combined in an amount of between about 15 and about 30 milligrams of calcium cations per gram of pectin.

6. The method of claim 3 in which the low methoxyl pectin material has approximately a five percent methoxyl content.

7. The method of claim 6 in which the calcium cations and liquid growth medium including pectin are combined in an amount of between about 15 and about 30 milligrams of calcium cations per gram of pectin.