Cell culture device

• US 4,242,459 A
• Filed: 11/02/1978
• Issued: 12/30/1980
• Est. Priority Date: 11/02/1978
• Status: Expired due to Term

• Current Assignee: Georg Panol, Peter D. Richardson, Pierre M. Galletti, William L. Chick
• Original Assignee: Georg Panol, Peter D. Richardson, Pierre M. Galletti, William L. Chick
• Inventors: Chick, William L., Galletti, Pierre M., Panol, Georg, Richardson, Peter D.
• Primary Examiner(s): Penland, R. B.
• Application Number:
  US05/957,303
• Time in Patent Office: 789 Days
• Field of Search: 195/127, 195/139, 195/1.7, 195/1.8, 128/DIG. 3, 210/321 B, 210/22, 422/44, 422/48, 435/284, 435/283, 435/286, 435/313, 435/818, 3/1
• US Class Current: 435/297.2
• CPC Class Codes: A01N 1/0263 Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
• CPC Class Codes: A61F 2/022 Artificial gland structures using bioreactors hemodialysis A61M1/16; hemofiltration A61M1/34; other extracorporeal blood treatment A61M1/36
• CPC Class Codes: C12M 23/06 Tubular C12M23/08, C12M23/16 take precedence
• CPC Class Codes: C12M 29/04 Filters; Permeable or porous membranes or plates, e.g. dialysis
• CPC Class Codes: C12M 29/10 Perfusion
• CPC Class Codes: Y10S 128/03 Heart-lung

Abstract

A cell culture device for use, e.g., as an artificial pancreas, featuring, in various aspects, a tightly wound spiral tube snugly fit in a cavity to form a cell culture compartment, the spiral being eccentrically offset in the cavity to reduce the volume of the compartment, a sloped passage in the body with an end of the tube extending through the passage to the innermost spiral without bending sharply, the ends of the spiral tube communicating with ports on the same side of the body, and a wall of the cavity facing the spiral and being spaced from the tube to form an end of the compartment to provide for simplified cell loading.

Claims

1. A cell culture device comprising,

a body having a generally circular fluid tight cavity therein,

said body having an inlet and an outlet and

a semi-permeable tube wrapped about itself to form coils in said cavity, said coils forming a flat spiral about a central axis,

the interior of said tube providing a fluid flow path for communicating with a biological fluid source,

said flat spiral coils positioned in said cavity between a bottom wall and a cover plate to form a cell culture compartment therebetween.

said tube being tightly wound in said flat spiral such that adjacent coils thereof touch each other to substantially eliminate dead space between said coils,

the ends of said tube extending through said inlet and said outlet, and

said cavity being shaped to entirely surround said coils and being just wide enough and high enough to accommodate said tightly wound coils, to further reduce dead space.

2. The cell culture device of claim 1 wherein,

said cavity is circular in cross section, and

said axis is eccentrically offset with respect to said cavity to reduce the volume of said compartment.

3. The cell culture device of claim 2 wherein,

said axis is eccentrically offset by one outside diameter of said tube.

4. The cell culture device of claim 1 further comprising,

a winding post positioned within said cavity,

said tube being wrapped around said post to form said coils.

5. The cell culture device of claim 4 wherein,

said cavity is circular, and

said winding post is a circular cylinder eccentrically offset with respect to said cavity by one outside diameter of said tube.

6. The cell culture device of claim 4 wherein,

said body has a port in the exterior surface thereof,

said post has a bore therein communicating with said compartment, and

said body has a passage therein communicating between said port and said bore,

whereby said compartment may be charged with cells through said port.

7. The cell culture device of claim 6 wherein,

said body has a vent communicating with said compartment,

whereby cells may be drawn into said compartment through said port by a vacuum applied to said compartment through said vent.

8. The cell culture device of claim 1 wherein,

a side of said body forming a wall of said cavity is optically clear,

whereby said compartment and said coils are viewable therethrough.

9. The cell culture device of claim 1 wherein,

said cover plate is removable to provide access to said cavity.

10. The cell culture device of claim 9 wherein,

said plate is optically clear,

whereby said compartment and said coils are viewable therethrough.

11. The cell culture device of claim 1 wherein

said inlet comprising a first port in an exterior side wall of said body,

a sloped first passage in said body extending beneath said coils, between said cavity and said port,

one end of said tube extending from the coil of said spiral closest to said axis, through said passage, to said first port,

said outlet comprising a second port in an exterior side wall of said body adjacent to said first port, and

a second passage in said body extending between said second port and said cavity,

the other end of said tube extending from the outermost coil of said tube, through said second passage, to said second port.

12. The cell culture device of claim 11 wherein,

said first and second passages lie in parallel planes.

13. The cell culture device of claim 11 wherein,

said first and second ports are on the same side of said body.

14. The cell culture device of claim 11 further comprising,

a winding post positioned within said cavity,

said tube being wrapped around said post to form said coils.

15. The cell culture device of claim 14 wherein, said post is circular in cross section, and further comprising,

a falciform element positioned between said post and the innermost coil of said tube to prevent the next adjacent coil of said tube from crossing over the innermost coil.

16. The cell culture device of claim 1 wherein said bottom wall of said flat spiral coils being spaced away from the coils to form a chamber in said compartment between said wall and said coils,

said body having an exterior surface and cell-charging port through said exterior surface and a passage communicating between said port and said chamber,

whereby said compartment may be charged with cells through said port, the cells distributing through said compartment from said chamber.

17. The cell culture device of claim 16 wherein said passage communicates with said chamber at the axis of said flat spiral coils,

whereby said cells distribute substantially radially.

18. The cell culture compartment of claim 16 further comprising,

a winding post positioned within said cavity,

said tube being wrapped around said post to form said coils,

said post having a bore therein, and

said passage communicating with said chamber through said bore.

19. The cell culture device of claim 16 wherein,

said bottom wall is spaced no more than 0.5 mm from said coils.

20. The cell culture device of claims 1 or 9 further comprising,

a mesh element positioned between said coils and said bottom wall of said cavity perpendicular to said central axis.