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Quantitative protein analysis by immunodiffusion

  • US 4,244,803 A
  • Filed: 11/27/1979
  • Issued: 01/13/1981
  • Est. Priority Date: 11/27/1979
  • Status: Expired due to Term
First Claim
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1. Apparatus for obtaining a quantitative measure of the concentration of a protein in an antigen sample, comprisingmeans for supporting and illuminating a plate carrying a precipitation zone due to immunoelectrophoresis of said antigen sample with an antibody specific to said protein,photoresponsive means for extracting a plurality of electrical signals representing the light intensity at positions on said plate which correspond to respective sets of position representing signals,and electronic means for supplying to said photoresponsive means sets of position representing signals corresponding to positions in selected respective spatial relationship to said zone, and for deriving from the resulting light intensity representing signals the corresponding value of a parameter which varies in characteristic manner with said protein concentration.

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