Test means and method for interference resistant determination of oxidizing substances

• US 4,247,297 A
• Filed: 02/23/1979
• Issued: 01/27/1981
• Est. Priority Date: 02/23/1979
• Status: Expired due to Term

• Current Assignee: Miles Laboratories, Inc. (Bayer AG)
• Original Assignee: Miles Laboratories, Inc. (Bayer AG)
• Inventors: White-Stevens, Rodric H., White, William I., Berti, Giovanni
• Primary Examiner(s): Marantz, Sidney
• Application Number:
  US06/014,693
• Time in Patent Office: 704 Days
• Field of Search: 23/230 B, 23/913, 23/901, 422/56, 252/408, 435/28, 435/4, 435/10, 435/14, 435/805
• US Class Current: 436/169
• CPC Class Codes: C12Q 1/28 involving peroxidase
• CPC Class Codes: C12Q 2326/32 3-Methyl-2-benzothiazolinone hydrazone hydrochloride hydrate, i.e. MBTH
• CPC Class Codes: C12Q 2326/50 Phenols; Naphthols; Catechols
• CPC Class Codes: Y10S 435/805 Test papers
• CPC Class Codes: Y10S 436/904 Oxidation - reduction indicators
• CPC Class Codes: Y10T 436/144444 Glucose

Abstract

Test means, such as a composition or device, method of making a test device and process for determination of at least one oxidizing substance, such as a peroxide, are disclosed. More particularly, the contemplated test means comprises a hydrazone and 8-amino-1-napthol-5,7-disulfonic acid (Chicago acid). Further provided is a test system for the determination of a constituent in a sample, having means responsive to the presence of said constituent in said sample to produce at least one oxidizing substance and a composition for determining said at least one oxidizing substance, wherein said composition comprises a hydrazone and 8-amino-1-napthol-5,7-disulfonic acid (Chicago acid). The test system is preferably of the type which determines peroxides formed from enzymatic conversion of constituents in biological fluids. When in the form of compositions the test means can optionally be incorporated with a carrier, such as a tablet or matrix, to provide a test device. The test system is highly sensitive to low levels of body fluid constituents to be detected, while also being highly resistant to interfering reducing substances, such as ascorbic acid, often present in body fluids.

Claims

1. Test means for the determination of an oxidizing substance which comprises an indicator system consisting essentially of a hydrazone and 8-amino-1-napthol-5,7-disulfonic acid.

2. The test means of claim 1 wherein said hydrazone is 3-methyl-2-benzothiazolinone hydrazone.

3. A test device for the determination of an oxidizing substance which comprises a carrier matrix and, incorporated therewith, the test means of claim 1.

4. A test device for the determination of an oxidizing substance which comprises a carrier matrix and, incorporated therewith, the test means of claim 2.

5. The test device of claim 3 wherein the carrier matrix is bibulous.

6. A test device for the determination of an oxidizing substance which comprises a tablet incorporated with the test means of claim 1.

7. A process for the preparation of a device for the determination of oxidizing substances which comprises incorporating a carrier matrix with the test means of claim 1.

8. The process of claim 7 wherein said incorporating is impregnating with a solution of said test means, followed by drying.

9. A process for determination of an oxidizing substance in a liquid sample which comprises contacting said sample with the test means of claim 1 and detecting any resultant response.

10. A process for determination of an oxidizing substance in a liquid sample which comprises contacting said sample with the test device of claim 3 and observing any resultant color change thereon.

11. In a test system for the determination of a constituent in a sample, having means responsive to the presence of said constituent in said sample to produce at least one oxidizing substance and having a composition for determining said at least one oxidizing substance, the improvement wherein said composition comprises an indicator system consisting essentially of a hydrazone and 8-amino-1-napthol-5,7-disulfonic acid.

12. The test system of claim 11 wherein said hydrazone is 3-methyl-2-benzothiazolinone hydrazone.

13. A test device which comprises a carrier matrix and, incorporated therewith, the test system of claim 11.

14. A test device which comprises a carrier matrix and, incorporated therewith, the test system of claim 12.

15. The device of claim 13 wherein the carrier matrix is bibulous.

16. A test device which comprises a tablet incorporated with the test system of claim 11.

17. A process for the preparation of a test device which comprises incorporating a carrier matrix with the test system of claim 11.

18. The process of claim 17 wherein said incorporating is impregnating with a solution of said test system, followed by drying.

19. A process for determination of a constituent in a liquid sample which comprises contacting said sample with the test system of claim 11 and observing any resultant color change.

20. A process for determination of a constituent in a liquid sample which comprises contacting said sample with the test device of claim 13 and observing any resultant color change.