Preservative solutions containing sodium dehydroacetate with or without sodium borate decahydrate and/or disodium edetate

• US 4,248,634 A
• Filed: 05/04/1979
• Issued: 02/03/1981
• Est. Priority Date: 05/26/1978
• Status: Expired due to Term

• Current Assignee: John Forester
• Original Assignee: John Forester
• Inventors: Forester, John
• Primary Examiner(s): Welsh, Maurice J.
• Application Number:
  US06/036,131
• Time in Patent Office: 641 Days
• Field of Search: 106/15.05, 252/408, 424/148, 424/153
• US Class Current: 106/15.05
• CPC Class Codes: A01N 2300/00 Combinations or mixtures of active ingredients covered by classes A01N27/00 - A01N65/48 with other active or formulation relevant ingredients, e.g. specific carrier materials or surfactants, covered by classes A01N25/00 - A01N65/48
• CPC Class Codes: A01N 43/16 with oxygen as the ring hetero atom
• CPC Class Codes: A01N 59/14 Boron; Compounds thereof
• CPC Class Codes: Y10T 436/101666 Particle count or volume standard or control [e.g., platelet count standards, etc.]
• CPC Class Codes: Y10T 436/105831 Protein or peptide standard or control [e.g., hemoglobin, etc.]
• CPC Class Codes: Y10T 436/107497 Preparation composition [e.g., lysing or precipitation, etc.]
• CPC Class Codes: Y10T 436/108331 Preservative, buffer, anticoagulant or diluent

Abstract

Sodium azide is used as a preservative or bacteriostatic agent of various solutions such as hypertonic phosphate solution, cleaning solutions used in electronic counting equipment, Sorensen'"'"'s phosphate buffers, lytic agents E and F and the like. Unfortunately, sodium azide, when combined with lead or copper in sink plumbing can produce explosive substances and also is capable of generating toxic gases. By using sodium dehydroacetate with or without sodium borate decahydrate and/or disodium edetate, the disadvantages are removed and at the same time the efficiency of the various solutions is not affected adversely.

Claims

1. A multi-purpose hypertonic phosphate solution for use in automated cell counters, as a cleaning solution in hemoglobinometry and cleaning solutions used on all types of automated cell counters with a lytic agent concentrate consisting essentially of:

(a) an osmotically balanced solution of sodium chloride, potassium chloride, sodium phosphate dibasic and sodium phosphate monobasic, and;

(b) sodium dehydroacetate, said solution being an aqueous electrolyte solution wherein the sodium dehydroacetate is bacteriostatic in nature.

2. The solution according to claim 1 which includes disodium edetate.

3. The solution according to claim 1 which includes sodium borate decahydrate.

4. The solution according to claim 2 which includes sodium borate decahydrate.

5. The solution according to claim 1 in which the approximate proportions by volume comprise sodium chloride 0.8% to 0.83%, sodium phosphate dibasic 0.170% to 0.175%, sodium phosphate monobasic 0.029% to 0.035%, potassium chloride 0.038% to 0.040%, and the sodium dehydroacetate 0.45% to 0.50%.

6. The solution according to claim 5 which includes disodium edetate in the proportion of approximately 0.1% by volume.

7. The solution according to claim 5 which includes sodium borate decahydrate in the proporation of 0.06% to 0.10%.

8. The solution according to claim 6 which includes sodium borate decahydrate in the proportion of 0.06% to 0.10%.

9. The solution according to claims 1, 2 or 3 together with a lytic agent concentrate, said lytic agent concentrate comprising approximately 3.2 grams of potassium cyanide, 78.0 grams of cetyl trimethyl ammonium bromide, approximately 110.0 ml of glycerol USP, with said solution making up the amount of one liter.

10. The solution according to claims 1, 2 or 3 together with a lytic agent concentrate, said lytic agent concentrate comprising approximately 0.25 grams of potassium cyanide, 20.0 grams of cetyl trimethyl ammonium bromide, with said solution making up a volume of one liter, the pH being adjusted to approximately 7.4 with concentrated phosphoric acid.

11. The solution according to claims 1, 2 or 3 together with a non-ionic surfactant selected from the group comprising Triton X-100, Brij 35 and Triton CF-54, said non-ionic surfactant comprising approximately 0.1% by volume of the total.