Test for assessing the unsaturated binding capacity of serum proteins which bind thyroid hormones

• US 4,252,782 A
• Filed: 09/29/1978
• Issued: 02/24/1981
• Est. Priority Date: 09/29/1978
• Status: Expired due to Term

• Current Assignee: Rhone-Poulenc Rorer Pharmaceuticals Incorporated (Sanofi )
• Original Assignee: Meloy Laboratories Incorporated
• Inventors: Bailey, Anne L.
• Primary Examiner(s): Padgett, Benjamin R.
• Assistant Examiner(s): Nucker, Christine M.
• Application Number:
  US05/947,056
• Time in Patent Office: 879 Days
• Field of Search: 424/1, 424/12, 23/230 B, 422/61
• US Class Current: 424/1.45
• CPC Class Codes: G01N 33/78 Thyroid gland hormones , e.g. T3, T4, TBH, TBG or their receptors
• CPC Class Codes: Y10S 436/804 Radioisotope, e.g. radioimmunoassay
• CPC Class Codes: Y10S 436/808 Automated or kit
• CPC Class Codes: Y10S 436/81 Tube, bottle, or dipstick

Abstract

An improved test for assessing the unsaturated binding capacity of serum proteins which bind thyroid hormones employs a clay-organic complex for adsorbing a free radioactive hormone fraction. The sorbent, an organically modified montmorillonite, provides better separation of thyroid states and may be employed in smaller amounts than prior sorbents employed in comparable tests.

Claims

1. In a test for assessing the unsaturated binding capacity of serum proteins which bind thyroid hormones and in which a radioactive thyroid hormone is admixed with a serum sample, free radioactive hormone is adsorbed by a sorbent added to the mixture, the adsorbed and non-adsorbed fractions are separated, and the radioactivity of the adsorbed or non-adsorbed fraction is determined, the improvement wherein the sorbent comprises a montmorillonite-ammonium complex.

2. A test in accordance with claim 1, wherein the complex is produced by adding an amine salt or a quaternary ammonium salt or base to a clay-water suspension and exchanging organic cations for exchangeable cations on the clay surfaces.

3. A test in accordance with claim 1, wherein the complex is selected from the group consisting of a triaalkylaryl ammonium smectite and a tetraalkyl ammonium smectite.

4. A test in accordance with claim 1, wherein the sorbent is added in the form of a dispersion.

5. A test in accordance with claim 4, wherein the complex is dispersed in a buffer.

6. A test in accordance with claim 5, wherein the pH of the buffer is between about 6.5 and about 10.7.

7. A test in accordance with claim 1, wherein the sorbent is added in a dispersion with a concentration in the range of from a fraction of a mg/ml to several mg/ml.

8. A test in accordance with claim 1, wherein the sorbent is added in a dispersion and is admixed with an agent for maintaining the complex in suspension.

9. A test in accordance with claim 8, wherein the agent is a guar gum.

10. In a test kit for measuring T3 uptake and including packaged units of T3 uptake normal human control serum and radioactive T3 buffer reagent, the improvement comprising, as part of the test kit, a packaged unit of a sorbent comprising an organically modified montmorillonite constituting a montmorillonite-ammonium complex.

11. A test kit in accordance with claim 10, wherein the sorbent comprises a complex selected from the group consisting of a trialkylaryl ammonium smectite and a tetraalkyl ammonium smectite.

12. A test kit in accordance with claim 10, wherein the organically modified montmorillonite is in a dispersion.

13. A test kit in accordance with claim 10, wherein the organically modified montmorillonite is dispersed in a buffer having a pH between about 6.5 and about 10.7.

14. A test kit in accordance with claim 13, wherein the concentration of the organically modified montmorillonite is in the range of from a fraction of a mg/ml to several mg/ml.

15. A test kit in accordance with claim 10, wherein the organically modified montmorillonite is admixed with a gum in a dispersion.

16. A test kit in accordance with claim 15, wherein the gum is a guar gum.