Method and apparatus for automated analysis of fluid samples

• US 4,253,846 A
• Filed: 11/21/1979
• Issued: 03/03/1981
• Est. Priority Date: 11/21/1979
• Status: Expired due to Term

• Current Assignee: Technicon Instruments Corporation
• Original Assignee: Technicon Instruments Corporation
• Inventors: Pelavin, Milton H., Smythe, William J., Isreeli, Jack
• Primary Examiner(s): Serwin, R. E.
• Application Number:
  US06/096,703
• Time in Patent Office: 468 Days
• Field of Search: 23/230 R, 23/230 B, 422/81, 422/82
• US Class Current: 436/53
• CPC Class Codes: G01N 35/08 using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
• CPC Class Codes: Y10T 436/118339 with formation of a segmented stream

Abstract

A sample analyzing system features a number of discrete sample segments continuously conveyed along a conduit. Each sample segment receives a precise aliquot of one or more reagents on a selective basis, and in any particular sequence. Selective injection of the reagent into the moving stream of sample segments allows for an increase in the efficiency and throughput of the sample processing.

Claims

1. A continuous system for analyzing a number of liquid samples, comprising:

a conduit defining a sample flow path;

means for conveying said liquid samples successively as sets of discrete sample segments and as a continuous stream along said flow path;

means disposed along said flow path for selectively introducing, in a variable controlled sequence, precise aliquots of different reagents into select ones of said sample segments in each of said sets to react said selected sample segments each in respect of at least a particular analyte;

means for controlling the sequence in which said various reagent aliquots are introduced into each of said sets; and

means disposed along said flow path for analyzing said samples.

2. The system of claim 1, further comprising means disposed along said flow path for detecting the flow of said sample segments along said flow path, said control means being responsive to said detecting means.

3. The system of claim 1, further comprising means for introducing said discrete sample segments along said flow path, and means associated with said sample introducing means for indicating the different analyses to be effected in respect of said sample segments in each of said sets, said controlling means being responsive to said indicating means.

4. The system of claim 3, wherein said sample introducing means is operative to segment each liquid sample to define a set equal to the number of different analytes to be determined in respect of said each liquid sample.

5. The system of claim 3, wherein said sample introducing means comprises means for aspirating said successive sample segments, each separated by at least an inert fluid segment.

6. The system of claim 3, wherein said sample introducing means comprises means for introducing an immiscible fluid along said flow path, said immiscible fluid preferentially wetting the surfaces of said conduit to the exclusion of said sample segments.

7. The system of claim 3, wherein said indicating means further comprises means for reading a label associated with each sample set.

8. The system of claim 7, wherein each label comprises a code for indicating the different analyses to be performed for each respective sample set.

9. The system of claim 1, wherein said reagent introducing means introduces aliquots of reagents which are generally but not limited to between 5% and 15% by volume of the sample segments inclusive of diluent.

10. The system of claim 1, wherein said sample analyzing means comprises a colorimeter.

11. The system of claim 10, further comprising means responsive to said detecting means for changing the operating wavelength of said colorimeter in respect of each sample segment, so as to correspond with the analyte to be analyzed in said each sample segment.

12. The system of claim 1, further comprising means for introducing a marker into said flow path in order to monitor the number of sample sets being conveyed along said flow path.

13. The system of claim 12, further comprising means disposed along said flow path for detecting said marker.

14. A method for quantitatively determining analytes in discrete liquid samples, said method comprising the steps of:

(a) flowing said liquid samples successively as a continuous stream through said conduit;

(b) dividing each of said liquid samples into a number of discrete segments equal, at least, to the number of analytes to be determined;

(c) introducing a controlled quantity of at least one reagent of a plurality of reagents into each of said sample segments in each of said sets on a selective basis to react said sample segments in each of said sets in respect of said different analytes; and

(d) analyzing said reacted sample segments in each of said sets in respect of said different analytes.

15. The method of claim 14, comprising the further step of separating successive ones of said sample segments by at least an inert fluid segment.

16. The method of claim 14, comprising the further step of introducing said reagent as said sample segments are flowed along said conduit.

17. The method of claim 14, comprising the further step of introducing two or more of said plurality of reagents in a plane or planes transverse of said conduit.

18. The method of claim 14, comprising the further step of introducing two or more of said plurality of reagents at separate points along said conduit.

19. The method of claim 14, comprising the further step of introducing an immiscible fluid to preferentially wet the surfaces of said conduit to the exclusion of said sample segments.

20. The method of claim 15, comprising the further step of introducing an immiscible fluid to preferentially wet the surfaces of said conduit to the exclusion of said sample segments and said inert fluid segments.

21. The method of claim 14, comprising the further steps of identifying the analytes to be determined in respect of each of said liquid samples, and controlling the introduction of said controlled quantity of reagents into said sample segments according to said analytes to be determined.

22. The method of claim 14, comprising the further steps of identifying the analytes to be determined in respect of each of said liquid samples, each of said liquid samples being divided into a number of sample segments, so as to define a set, equal to said number of analytes to be determined.

23. Apparatus for quantitatively determining one or more analytes in a plurality of discrete samples, comprising:

a conduit;

means for dividing said samples into sets of discrete sample segments;

means for flowing said sets of discrete sample segments successively as a continuous stream through said conduit, said successive sample segments being separated by at least an inert fluid segment;

a reagent injection station disposed along a portion of said conduit, said injection station including means to introduce, on a selective basis, aliquots of reagents into sample segments passing along said conduit portion;

means for controlling said injection station, on a selective basis, to introduce selected reagents into said conduit portion, to mix with a particular one of said sample segments; and

an analyzing station disposed downstream of said injection station for analyzing said sample segments.

24. The apparatus of claim 23, including means for introducing an immiscible fluid along said conduits, said immiscible fluid preferentially wetting the surfaces of said conduit to the exclusion of said sample segments.

25. The method of claim 23, comprising the further step of introducing an immiscible fluid to preferentially wet the surfaces of said conduit to the exclusion of said sample segments and said inert fluid segments.

26. The apparatus of claim 23, further comprising detection means disposed along said conduit for locating sample segments to be injected with reagent.

27. The apparatus of claim 23, wherein said reagent station comprises a plurality of poppet valves disposed about said conduit, each of said valves having a tip which forms part of the conduit wall.

28. The apparatus of claim 27, wherein each of said tips is substantially flush or slightly convex with respect to said conduit wall.

29. The apparatus of claim 27, wherein said plurality of valves are disposed circumferentially about said conduit.

30. The apparatus of claim 23, wherein said tips of said poppet valves are comprised of the same material as said conduit wall.

31. The apparatus of claim 30, further including means for introducing an immiscible fluid along said conduit, said immiscible fluid preferentially wetting the surfaces of said conduit and the tips of said poppet valves to the exclusion of said sample segments.