Method and apparatus for the measurement of glucose content
First Claim
1. In a method of measuring glucose content, in which the glucose content of a liquid sample is analyzed by contacting the glucose-containing liquid sample with glucose oxidase to produce hydrogen peroxide and the amount of hydrogen peroxide produced is determined, the improvement comprising pretreating the liquid sample with the salt form of a strongly basic anion exchange resin and the salt form of a strongly acidic cation exchange resin, then contacting the sample with glucose oxidase to produce hydrogen peroxide in amount proportional to the glucose present in the sample and determining the amount of hydrogen peroxide produced through detection electrodes to which an alternating voltage of rectangular wave form is applied thereby generating an oxidation current proportional to the amount of hydrogen peroxide present in the sample located at the detection electrodes and detecting said oxidation current.
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Abstract
A method and apparatus for measuring the glucose content in a liquid sample by passing the sample consecutively through a column of at least one ion exchange resin and a column of immobilized glucose oxidase and then determining the amount of hydrogen peroxide produced by contacting the sample with glucose oxidase.
82 Citations
11 Claims
- 1. In a method of measuring glucose content, in which the glucose content of a liquid sample is analyzed by contacting the glucose-containing liquid sample with glucose oxidase to produce hydrogen peroxide and the amount of hydrogen peroxide produced is determined, the improvement comprising pretreating the liquid sample with the salt form of a strongly basic anion exchange resin and the salt form of a strongly acidic cation exchange resin, then contacting the sample with glucose oxidase to produce hydrogen peroxide in amount proportional to the glucose present in the sample and determining the amount of hydrogen peroxide produced through detection electrodes to which an alternating voltage of rectangular wave form is applied thereby generating an oxidation current proportional to the amount of hydrogen peroxide present in the sample located at the detection electrodes and detecting said oxidation current.
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9. An apparatus for measuring glucose content, which involves:
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(a) an ion exchange resin column section of at least one column of the salt form of a strongly basic anion exchange resin and the salt form of a strongly acidic cation exchange resin, the columns of said column section being in series when there is more than one column in the section, and an inlet conduit and an outlet conduit joined to the column section for passing a glucose-containing liquid sample therethrough, (b) means for supplying a buffer solution to said liquid sample while it is passing through said outlet conduit joined to the ion exchange resin column section, said means including a buffer solution conduit joined to the outlet conduit at a location between its termini, (c) a column of immobilized glucose oxidase attached to the terminus of said outlet conduit distant from the ion exchange resin column section for passing therethrough the liquid sample, after it has left the column of said ion exchange resin and then has been diluted with a buffer solution, to produce hydrogen peroxide by contacting the liquid sample with glucose-oxidase, (d) an amperometric detector with detection electrodes to which an alternating voltage of rectangular wave form is applied, for determining the amount of hydrogen peroxide contained in the liquid sample which has left the column of immobilized glucose oxidase and a conduit joining the glucose oxidase to the amperometric detector for delivering the liquid sample to the amperometric detector. - View Dependent Claims (10, 11)
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Specification