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Fluorescence quenching with immunological pairs in immunoassays

  • US 4,261,968 A
  • Filed: 05/10/1979
  • Issued: 04/14/1981
  • Est. Priority Date: 05/10/1979
  • Status: Expired due to Term
First Claim
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1. Reagent for determining the presence or amount of a ligand comprising two chromophores, which are a fluorescer-quencher pair, the amount of fluorescer being within quenching distance of said quencher being affected by the presence or amount of ligand, wherein one chromophore is bonded to ligand and the other chromophore is bonded to antiligand capable of specific non-covalent binding to said ligand.

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