Clinical analytical system

• US 4,264,560 A
• Filed: 12/26/1979
• Issued: 04/28/1981
• Est. Priority Date: 12/26/1979
• Status: Expired due to Term

• Current Assignee: Samuel Natelson
• Original Assignee: Samuel Natelson
• Inventors: Natelson, Samuel
• Primary Examiner(s): Serwin, Ronald
• Application Number:
  US06/106,550
• Time in Patent Office: 489 Days
• Field of Search: 422/56, 422/57, 422/58, 422/61, 422/66, 422/81, 422/82, 23/901, 356/246
• US Class Current: 422/417
• CPC Class Codes: G01N 35/00009 provided with a sample supporting tape, e.g. with absorbent zones
• CPC Class Codes: G01N 35/1079 with means for piercing stoppers or septums

Abstract

An arrangement for chemical analysis of a small quantity of sample wherein a specimen of a small size is passed through a porous distribution first medium onto a reagent-containing second medium is disclosed. The reagent-containing second medium is a thin, flat, liquid-impervious medium. A reagent is encapsulated upon the second medium as a flat, liquid-phase surface. The first and second mediums are so arranged and disposed that when firmly pressed together, the encapsulated liquid reagent will be liberated and the specimen will be distributed through the first medium onto the liquid-phase liberated reagent where the subsequent reaction of the liquid-phase reagent and the specimen can then be identified by reading means.

Claims

1. An arrangement for the analysis of small quantities of chemical samples, comprising in combination:

(a) a sample distributor flat first component for defining an area to receive the specimen to be analyzed;

(b) a pervious layer second component in operational contact with said first component, said second component including positional means for positioning a section thereof of about the size of said first component;

(c) a liquid phase reagent container third component including a defined thin disc-shaped processing zone in contact with said second component having in said processing zone at least one hollow vessel, said hollow vessel having inlet and outflow channels, said inlet channel being on one side and said outflow channel being on the other side of said third component so that a liquid reagent may be introduced into said processing zone to form a thin film of a reagent, whereby the component being assayed may move through the pervious layer from the sample distributor and be evenly dispersed in the thin film of reagent for analysis.

2. An arrangement as claimed in claim 1 including pump means coupled to said inlet channel to pump a liquid phase reagent thereinto, and wash out said processing zone for reuse.

3. An arrangement as claimed in claim 1 wherein said third component has a plurality of processing zones defined therein, said first component covering said zones so as to distribute the sample to be analyzed in each zone through said pervious layer second component for the analysis of a plurality of the different contents of said sample.

4. An arrangement as claimed in claim 3 including optical photocell readout means disposed to readout the sample analyzed in each of said zones.

5. An arrangement as claimed in claim 4 wherein said readout means is a readout station whereat a sample specimen is presented for evaluation thereat on said defined thin flat processing zone, said evaluation being provided as the response of said photocell in terms of concentration of the chemical components being assayed, said arrangement including optical fiber means having an outer bundle and an inner bundle, said inner and outer bundles both having one end disposed opposite said read-out station, said bundles then separating, one of said bundles having its other end disposed to receive sample illuminating light beams from a light source, said other bundle having its other end disposed to carry a beam reflected by the sample to said photocell.

6. An apparatus as claimed in claim 5 wherein said one of said bundles is the inner bundle.

7. An apparatus as claimed in claim 5 wherein said one of said bundles is the outer bundle.

8. An arrangement for use in an apparatus for the chemical analysis of a specimen which is to be deposited in a confined area of a sample receiving first flat medium and wherein a component of said specimen is passed through a porous second medium to a reagent-containing third medium, the improvement in said arrangement wherein the third medium is a thin layer of the reagent in the liquid phase disposed in a confined area of about the same size as the confined area of the first medium.

9. An arrangement as claimed in claim 8 wherein the area of the porous medium is larger than the area of the sample receiving first medium.

10. An arrangement as claimed in claim 8 wherein said reagent comprises a first reagent means encapsulated in said confined area and at least one second reagent means capsule disposed over the first encapsulated reagent means at said defined area.

11. An arrangement as claimed in claim 10 including a spacer disposed over said third medium, said defined area having a cylindrical container defined in said spacer, and, inlet and outlet narrow channels leading to said container to feed liquid phase reagent to the container and flow out the excess reagent.

12. A liquid phase reagent analytical arrangement comprising a defined thin disc-shaped processing zone fitted with inlet and outlet channels, open at the top, in contact with a flat medium, closing the top of said disc-shaped processing zone so that a sample evenly distributed over said flat medium may be analyzed by transfering said sample evenly to a liquid reagent in said zone, said reagent being formed by said zone into a thin film.