Apparatus for determining the concentration of the components of a sample

• US 4,267,572 A
• Filed: 04/03/1979
• Issued: 05/12/1981
• Est. Priority Date: 04/08/1978
• Status: Expired due to Term

• Current Assignee: Bodenseewerk Perkin-Elmer GmbH (Revvity, Inc.)
• Original Assignee: Bodenseewerk Perkin-Elmer & Company
• Inventors: Witte, Wolfgang
• Primary Examiner(s): Wise, Edward J.
• Application Number:
  US06/026,710
• Time in Patent Office: 770 Days
• Field of Search: 364/498, 364/499, 356/325, 356/319-324
• US Class Current: 702/23
• CPC Class Codes: G01J 3/28 Investigating the spectrum using colour filters G01J3/51

Abstract

Apparatus for determining the concentration of the various components of a sample, which includes a spectrophotometer for receiving a sample to be analyzed and performing extinctions of the sample at a plurality of preselected wavelengths to form a sample spectrum; memory devices for storing a standard spectra of each of the individual components; circuitry for weighting the sample spectrum at different wavelengths in conformity with each of the standard spectra and providing estimated values of the concentrations of the components from the sample spectra thus weighted; circuitry for reconstructing the sample spectrum as a linear combination of the estimated values of the concentrations and the standard spectra; a subtractor for forming a difference spectrum from the sample spectrum and the reconstructed sample spectrum; circuitry for weighting the difference spectrum at different wavelengths in conformity with each of the standard spectra and providing corrective values of the concentrations of the components from the difference spectrum thus weighted, and means for reconstructing the difference spectrum as a linear combination from the corrective values of the concentrations and the standard spectra; a subtractor for forming a second difference spectrum from the first difference spectrum and the reconstructed difference spectrum; updating means for correcting the estimated values by the corrective values, and a readout device for reading out the corrected values of the concentrations of the components.

Claims

1. Apparatus for determining the concentration of the various components of a sample comprising, in combination,

a spectrophotometer for receiving a sample to be analyzed and performing a fixed number of extinctions of the sample at fixed wavelengths to form a sample spectrum;

means for forming and storing spectra in standardized form of each of the individual components, by the extinction of standard solutions of the components being looked for, having said fixed number of extinction values measured at said fixed wavelengths;

means for weighting the sample spectrum at different wavelengths in conformity with each of the standard spectra and providing estimated values of the concentrations of the components from the sample spectra thus weighted;

means for reconstructing the sample spectrum as a linear combination of the estimated values of the concentrations and the standard spectra;

means for forming a difference spectrum from the sample spectrum and the reconstructed sample spectrum;

means for weighting the difference spectrum at different wavelengths in conformity with each of the standard spectra and providing corrective values of the concentrations of the components from the difference spectrum thus weighted;

means for reconstructing the difference spectrum as a linear combination of the corrective values of the concentrations and the standard spectra;

means for forming a second difference spectrum from the first difference spectrum and the reconstructed difference spectrum;

means for correcting the estimated values by the corrective values; and

means for reading out the corrected values of the concentration of the components.

2. Apparatus for determining the concentration of the various components of a sample according to claim 1, wherein said means for weighting the sample spectrum comprises means for integrating the product of the sample spectrum and a respective one of the standard spectra through a predetermined wavelength range.

3. Apparatus for determining the concentration of the various components of a sample comprising, in combination,

a spectrophotometer for receiving a sample to be analyzed and performing a fixed number of extinctions of the sample at fixed discrete wavelengths to form a sample spectrum;

means for forming and storing a spectra in standardized form of each of the individual components, by the extinction of standard solutions of the components being looked for, having said fixed number of extinction values measured at said fixed discrete wavelength;

means for weighting the sample spectrum at different wavelengths in conformity with each of the standard spectra and providing estimated values of the concentrations of the components from the sample spectra thus weighted, said last named means including means for summing the products of the extinctions of the sample spectrum and the standard spectra;

means for reconstructing the sample spectrum from the extinction values for the predetermined discrete wavelengths as a linear combination of the associated values of the standard spectra and of the estimated values of the concentration;

means for forming a difference spectrum from the differences of the extinctions of the sample spectrum and the associated computed extinction values of the reconstructed sample spectrum at the preselected discrete wavelengths;

means for weighting the difference spectrum at different wavelengths in conformity with each of the standard spectra and providing corrective values of the concentrations of the components from the difference spectrum thus weighted;

means for reconstructing the difference spectrum as a linear combination from the corrective values of the concentrations and the standard spectra;

means for forming a second difference spectrum from the first difference spectrum and the reconstructed difference spectrum;

means for correcting the estimated values by the corrective values; and

means for reading out the corrected values of the concentration of the components.

4. Apparatus for determining the concentration of the various components of a sample according to claim 3, wherein said means for forming and storing a standard spectra of each of the individual components includes magnetic cards.