Method and kit for separation of glycoproteins
First Claim
1. The method of separating glycoproteins from nonglycosylated proteins in a mixture which comprises bringing said mixture into contact with reactive agent comprising a dihydroxyboryl group bonded to a support to form a glycoprotein-dihydroxyboryl complex, and separating said complex from said mixture.
4 Assignments
0 Petitions
Accused Products
Abstract
Glycoproteins, especially glycosylated hemoglobins, are separated from non-glycosylated proteins, by complexing with a dihydroxyboryl reactive agent. The resulting complex can be separated physically from the non-glycosylated protein, making possible the determination of glycosylated hemoglobin content of lysed blood; a reactive agent and a kit are described for use in such determination and as a monitor in the control of diabetic treatment. The invention can also be used preparatively to isolate glycoproteins.
98 Citations
8 Claims
- 1. The method of separating glycoproteins from nonglycosylated proteins in a mixture which comprises bringing said mixture into contact with reactive agent comprising a dihydroxyboryl group bonded to a support to form a glycoprotein-dihydroxyboryl complex, and separating said complex from said mixture.
- 4. The method of assaying blood for glycosylated hemoglobin which comprises lysing a sample of blood, separating cellular debris from said lysed sample, buffering said lysed sample to a pH in the range between about 7.5 and 9.0, bringing said lysed sample into contact with a selective agent comprising a dihydroxyboryl group covalently bonded to an agarose support to form a complex of said dihydroxyboryl group with glycosylated hemoglobin in said sample, separating said complex from said sample, and determining the amount of glycosylated hemoglobin in said complex.
- 6. A kit for assaying the glycosylated hemoglobin content of a blood sample comprising as components (1) a reactive agent for glycosylated hemoglobin comprising a dihydroxyboryl group bonded to a support, (2) a buffer capable of maintaining the pH of said sample of blood at a value in the range between about 7.5 and 9.0, and (3) a supply of lysing agent for lysing said blood sample.
Specification