Apparatus for measuring membrane characteristics of vesicles

• US 4,271,001 A
• Filed: 03/15/1979
• Issued: 06/02/1981
• Est. Priority Date: 03/23/1978
• Status: Expired due to Term

• Current Assignee: Asahi Kasei Kogyo Kabushiki Kaisha (Asahi Kasei Corporation)
• Original Assignee: Asahi Kasei Kogyo Kabushiki Kaisha (Asahi Kasei Corporation)
• Inventors: Kobayashi, Hidehiko, Kimura, Takeo, Imafuku, Hiroshi
• Primary Examiner(s): Kaplan, G. L.
• Application Number:
  US06/020,688
• Time in Patent Office: 810 Days
• Field of Search: 204/195 B, 204/195 R, 204/195 M, 204/1 T, 324/29
• US Class Current: 204/406
• CPC Class Codes: G01N 33/48735 Investigating suspensions of cells, e.g. measuring microbe concentration by chemical means C12Q1/04; colony counters C12M1/34; concentration of particle suspensions in general G01N15/06

Abstract

Vesicles are suspended in a lipophilic ion solution and the lipophilic ion concentration is changed by the permeation of the lipophilic ions into the vesicles. An electrode selectively responsive to the lipophilic ions is immersed in the suspension to detect the time-change rate of the electrode potential, thereby to obtain a change in the lipophilic ion concentration with respect to time, from which are measured membrane characteristics of the vesicles, such as the permeability of a vesicular membrane to the lipophilic ions.

Claims

1. An apparatus for measuring membrane characteristics of vesicles and comprising:

p1 vessel means for containing vesicles and a solution of lipophilic ion;

electrode means, selectively responsive only to the lipophilic ion, for producing an output indicative of lipophilic ion concentration;

arithmetic unit means, electrically connected with said electrode means, for calculating the time-change rate of the detected output from said electrode means and also for calculating the permeability coefficient of the vesicular membrane for the lipophilic ion and/or the membrane potential of the vesicular membrane from said output of said electrode means, from said time-change rate, and from the following input data;

the volume and temperature of the solution, and the total volume and the total surface area of the vesicles suspended in the solution; and

input means for inputting said input data to said arithmetic unit means.

2. The apparatus according to claim 1 wherein said arithmetic unit means is digital arithmetic unit means.

3. The apparatus according to claim 1, wherein there is provided injector means for injecting a constant volume of the vesicles or lipophilic ion solution into the vessel.

4. The apparatus according to claim 3, wherein there is provided trigger signal generator means for applying to the arithmetic unit means an electric signal synchronized with the injecting operation of the injector means.

5. The apparatus according to claim 1, wherein there is provided a constant-temperature bath for holding the vessel means at a constant temperature.

6. The apparatus according to claim 1, wherein the vessel means and the electrode means are disposed in a shield box for cutting off electromagnetic noises.

7. The apparatus according to claim 1, wherein there is provided a recorder supplied with the detected output in analog form for recording it.