Filling for optodes

• US 4,272,484 A
• Filed: 09/05/1979
• Issued: 06/09/1981
• Est. Priority Date: 12/27/1978
• Status: Expired due to Term

• Current Assignee: Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften e.V. (Max-Planck-Gesellschaft zur Frderung der Wissenschaften e)
• Original Assignee: Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften e.V. (Max-Planck-Gesellschaft zur Frderung der Wissenschaften e)
• Inventors: Lubbers, Dietrich W.
• Primary Examiner(s): Serwin, Ronald
• Application Number:
  US06/072,780
• Time in Patent Office: 643 Days
• Field of Search: 23/232 R, 23/232 E, 23/230 B, 422/57, 422/52, 422/55, 422/86, 422/91, 422/68, 422/58, 422/50, 356/39, 356/85, 356/246, 356/436, 356/441, 204/19 SM, 204/19 P
• US Class Current: 422/82.07
• CPC Class Codes: G01N 21/77 by observing the effect on a chemical indicator
• CPC Class Codes: G01N 31/22 using chemical indicators G01N31/02 takes precedence
• CPC Class Codes: G01N 33/521 Single-layer analytical elements
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings

Abstract

In order to improve the selectivity of an indicator chamber or optode conting of an indicator and a membrane impermeable to the indicator and surrounding it, for use in the measurement of concentration of component particles by means of an optical light measurement system including a light source, a light receiver and a readout device, a substance which reacts with the particle to be measured is provided within the indicator chamber, and the reaction product then modifies the optical properties of the indicator.

Claims

1. In an indicator chamber (optode) comprising an indicator and a membrane surrounding said indicator and impermeable to it, for measurement of concentration of particles by means of a light measurement system including a light source, a light receiver and readout means, the improvement comprising providing in a filling within said indicator chamber a substance which reacts with said particle to be measured and which generates through said reaction a reaction product which modifies the optical properties of said indicator, thereby allowing the preparation of highly selective indicator chambers with a broad range of applicability.

2. An optode as defined in claim 1, wherein said substance is an enzyme.

3. An optode as defined in claim 2, wherein said enzyme is glucose oxidase.

4. An optode as defined in claim 1, wherein said substance is an antibody.

5. An optode as defined in claim 1, wherein said membrane consists of more than one layer.

6. An optode as defined in claim 1, wherein said membrane is provided with a carrier for selective transport of said particle to be measured.

7. An optode as defined in claim 1, wherein said substance is fixed into a carrier matrix.

8. An optode as defined in claim 1, wherein said chamber is in the form of a flat surface.

9. An optode as defined in claim 1, wherein said chamber is in the form of a capsule.

10. A optode as defined in claim 1, wherein a first chamber is provided within a second chamber which surrounds it.

11. An optode as defined in claim 10;

further comprising additional chambers surrounding said first chamber.

12. An optode as defined in claim 1, wherein a substance which binds said particle to be measured is provided within said chamber.

13. An optode as defined in claim 1, wherein a reference indicator is additionally provided.