Membranes for optodes

• US 4,272,485 A
• Filed: 09/05/1979
• Issued: 06/09/1981
• Est. Priority Date: 12/27/1978
• Status: Expired due to Term

• Current Assignee: Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften e.V. (Max-Planck-Gesellschaft zur Frderung der Wissenschaften e)
• Original Assignee: Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften e.V. (Max-Planck-Gesellschaft zur Frderung der Wissenschaften e)
• Inventors: Lubbers, Dietrich W.
• Primary Examiner(s): Serwin, Ronald
• Application Number:
  US06/072,781
• Time in Patent Office: 643 Days
• Field of Search: 23/232 R, 23/232 E, 23/230 B, 422/57, 422/52, 422/55, 422/86, 422/91, 422/68, 422/58, 422/50, 356/39, 356/85, 356/246, 356/436, 356/441, 435/299, 204/195 M, 204/195 P
• US Class Current: 422/82.06
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 31/22 using chemical indicators G01N31/02 takes precedence
• CPC Class Codes: G01N 33/521 Single-layer analytical elements

Abstract

In an indicator chamber (e.g., optode) arrangement including an indicator d a membrane which surrounds it and is impermeable to it, a carrier substance is provided within said membrane for transport of particles to be measured into the indicator chamber. Said carrier is mobile within said membrane. This improvement allows for the preparation of highly selective indicator arrangements and broadens the range of applicability of the method of measurement.

Claims

1. In an indicator arrangement comprising an indicator and a membrane forming a chamber surrounding said indicator and impermeable to it, for determination of concentrations of particle components, the improvement comprising providing within said membrane a carrier which is mobile within said membrane and which transports said particles through said membrane.

2. An indicator arrangement as defined in claim 1, wherein said measurement is effected by means of a light measurement system, including a light source, a light receiver and readout means.

3. An indicator arrangement as defined in claim 1, wherein said measurement is effected by means of an x-ray analysis system including an electron source, and said particles are heavy molecules.

4. An indicator arrangement as defined in claim 1, wherein said carrier is electrically charged.

5. An indicator arrangement as defined in claim 1, wherein said carrier is electrically neutral.

6. An indicator arrangement as defined in claim 1, wherein a substance for binding said particles is provided within said chamber formed by said membrane.

7. An indicator arrangement as defined in claim 1, wherein said membrane is lipophilic.

8. An indicator arrangement as defined in claim 1, wherein said membrane is hydrophilic.

9. An indicator arrangement as defined in claim 1, wherein said carrier is in liquid form embedded with a solid matrix of suitable porosity for said particles.

10. An indicator arrangement as defined in claim 1, wherein more than one membrane is employed, said membranes being provided with different carriers for transport of said particles.

11. An indicator arrangement as defined in claim 1, wherein said chamber is in the form of a flat surface.

12. An indicator arrangement as defined in claim 1, wherein said chamber is in the form of a capsule.

13. An indicator arrangement as defined in claim 1, wherein a selective indicator is employed.

14. An indicator arrangement as defined in claim 1, wherein a substance which reacts with said particles is provided within said chamber, said reaction producing reaction products which then modify the optical properties of said indicator.

15. An indicator arrangement as defined in claim 1, wherein a first chamber is provided within a second chamber which surrounds it.

16. An indicator arrangement as defined in claim 15;

further comprising additional enveloping membrane chambers.