Magnetic attraction transfer process for use in solid phase radioimmunoassays and in other assay methods

• US 4,272,510 A
• Filed: 04/26/1976
• Issued: 06/09/1981
• Est. Priority Date: 04/26/1976
• Status: Expired due to Term

• Current Assignee: Kendall O. Smith, Warren D. Gehle
• Original Assignee: Kendall O. Smith, Warren D. Gehle
• Inventors: Smith, Kendall O., Gehle, Warren D.
• Primary Examiner(s): Pianalto, Bernard D.
• Application Number:
  US05/680,506
• Time in Patent Office: 1,870 Days
• Field of Search: 427/47, 427/127, 424/1, 424/16, 424/12, 23/230 B, 23/253 TP, 23/259
• US Class Current: 427/598
• CPC Class Codes: B03C 1/0332 using permanent magnets
• CPC Class Codes: B03C 1/0335 using coils
• CPC Class Codes: B03C 1/286 disposed at the inner circumference of a recipient, e.g. magnetic drain bolt
• CPC Class Codes: B03C 2201/18 Magnetic separation whereby the particles are suspended in a liquid
• CPC Class Codes: B03C 2201/26 for use in medical applications
• CPC Class Codes: G01N 33/54326 Magnetic particles
• CPC Class Codes: G01N 35/0098 involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C
• CPC Class Codes: Y10S 436/804 Radioisotope, e.g. radioimmunoassay
• CPC Class Codes: Y10S 436/807 Apparatus included in process claim, e.g. physical support structures
• CPC Class Codes: Y10S 436/809 Multifield plates or multicontainer arrays

Abstract

A process and devices for transferring, by magnetic attraction, antigen-antibody adsorbent materials from one reaction mixture to another, and to facilitate an efficient rinsing of these materials, and for transferring adsorbent materials used in other assay methods.

Claims

1. A process, for use in solid phase immunoassays, for simultaneously transferring a plurality of antigen-antibody adsorbent units from one location to another, comprising the steps of providing a plurality of units each comprising a core containing ferromagnetic material, said core covered with at least one coating for preventing chemical interactions between said core and aqueous chemical solutions and for adsorbing antigens or antibodies on the outer surface thereof, placing each of said units in a separate receptacle containing an appropriate liquid and then simultaneously removing said units from said receptacles by magnetic attraction by the use of a transfer device having a plurality of probes of magnetic material which are spatially oriented with respect to the receptacles.

2. The process according to claim 1, wherein each of said units includes an inner coating for preventing chemical interactions between said core and the aqueous chemical solutions and an exterior coating for absorbing antigens or antibodies on said outer surface thereof.

3. The process according to claim 2, wherein said exterior coating comprises an adsorbing coat selected from the group consisting of polycarbonate, polyethylene, polystyrene, polypropylene, acrylics, glass and chromatographic substances.

4. The process according to claim 2, wherein said inner coating includes a stable metal selected from the group consisting of nickel, gold, zinc, chromium, cadmium and copper, for effectively sealing said core from penetration and chemical interaction with aqueous chemical solutions.

5. The process according to claim 4, wherein said exterior coating comprises an adsorbing coat selected from the group consisting of polycarbonate, polyethylene, polystyrene, polypropylene, acrylics, glass and chromatographic substances.

6. The process according to claim 2, wherein said inner coating comprises waterproof paint material for effectively sealing said core from penetration and chemical interaction with the aqueous chemical solutions.

7. The process according to claim 6, wherein said exterior comprises an adsorbing coat selected from the group consisting of polycarbonate, polyethylene, polystyrene, polypropylene, acrylics, glass and chromatographic substances.

8. The process according to claim 1, wherein said probes are supported by a body of non-ferrous material.

9. The process according to claim 8, wherein said support body is at least partially coated with a material capable of reducing or preventing adsorption of antibodies or antigens to its surface and of preventing degradation of said support body during exposure to aqueous chemical solutions used in the immunoassay process.

10. The process according to claim 8, wherein said probes are disposed flush with a surface of said support body.

11. The process according to claim 10, further including the step of releasing said units from said probes by sliding said surface of said support body relative to receptacles to cause said adhering unit to be released.

12. The process according to claim 8, wherein said probes project outwardly of said support body.

13. The process according to claim 12, further including the step of releasing said units from said probes by providing a release member surrounding each of said probes outwardly of said support body and moving said release members along said probes in a direction outwardly of said support body to cause said adhering units to be released.

14. The process according to claim 1, wherein each of said probes comprises an electromagnet connected to an electric source.

15. The process according to claim 14, further including the step of releasing said units from said probes by interrupting the connection to said electric source to cause said adhering units to be released.

16. The process according to claim 1, wherein said core of said units are composed of magnetic material, and said units are released from said probes by magnetically attracting said units.

17. The process according to claim 1, wherein the transferring of said units by magnetic attraction is facilitated by axially aligning said support body and said receptacles.

18. The process according to claim 11, wherein said releasing step further includes funneling each of said units into receptacles upon sliding said surface of said support body relative to said receptacles.

19. The process according to claim 1, further including the steps of releasing said units which magnetically adhere to said probes into other receptacles, repeating the transfer of said units as required in the radioimmunoassay and then transferring and releasing said units into receptacles for counting in an appropriate instrument for measuring radiation.