Cystic fibrosis detection method

• US 4,273,869 A
• Filed: 04/16/1979
• Issued: 06/16/1981
• Est. Priority Date: 04/16/1979
• Status: Expired due to Term

• Current Assignee: Regents of The University of Minnesota (University of Minnesota)
• Original Assignee: Regents of The University of Minnesota (University of Minnesota)
• Inventors: Shapiro, Burton L.
• Primary Examiner(s): Therkorn, Ernest G.
• Application Number:
  US06/030,179
• Time in Patent Office: 792 Days
• Field of Search: 424/2, 424/7, 435/4, 435/26, 435/805, 435/810
• US Class Current: 435/26
• CPC Class Codes: C12Q 1/32 involving dehydrogenase
• CPC Class Codes: G01N 33/5091 for testing the pathological state of an organism
• CPC Class Codes: Y10S 435/81 Packaged device or kit

Abstract

A method of diagnosing cystic fibrosis, identifying carriers for cystic fibrosis, and non-carriers or "normal" persons. Heretofore, no carrier or prenatal detection procedure for cystic fibrosis existed. The detection method is based on the discovery of the genetic abnormality or biochemical defect in cystic fibrosis, which occurs in mitochondria, minute bodies found in the cytoplasma of most cells which are the principal energy source of the cell and contain the cytochrome enzymes of terminal electron transport. The method comprises an assay carried out on preparations derived from human cells possessing mitochondria. The assay may be a kinetic assay of the enzyme complex of the energy conserving site of the mitochondrial electron transport system of the cells or an assay of mitochondrial activity governed by the enzyme complex. The assay is then evaluated by comparison with standards established as the result of similar assays of cells of other subjects of known condition. The assays provide determination of characteristics which differ in the three types of individuals (normal, carrier and affected) and provide a basis for their distinction.

Claims

1. A method for the diagnosis of cystic fibrosis, the detection of non-symptomatic carriers of the disease and the identification of non-affected persons, which method comprises:

(A) obtaining a sample of cells possessing mitochondria from a human subject,

(B) preparing a homogenate of the cells,

(C) performing an assay on the homogenate of cells which measures the mitochondrial activity of the cells, and

(D) evaluating the assay by comparing the measurements with standards established as a result of similar assays of other subjects of known cystic fibrosis condition.

2. A method according to claim 1 wherein said assay is a kinetic assay of the enzyme complex of an energy conserving site of the mitochondrial electron transport system of the cells.

3. A method according to claim 2 wherein said enzyme is the enzyme of the first energy conserving site, NADH dehydrogenase.

4. A method according to claim 2 wherein said assay is a determination of the affinity of the enzyme for its substrate.

5. A method according to claim 4 wherein enzyme affinity for its substrate is measured at at least two of three pH values representing least alkaline conditions, more alkaline conditions and most alkaline conditions.

6. A method according to claim 5 further characterized in that said pH values are about 8.0, 8.3 and 8.6.

7. A method according to claim 2 wherein said assay is a determination of pH optimum for enzyme activity.

8. A method according to claim 7 wherein enzyme activity is measured at at least two of three pH values representing least alkaline conditions, more alkaline conditions and most alkaline conditions.

9. A method according to claim 8 wherein said pH values are about 8.0, 8.3 and 8.6.

11. A method according to claim 2 wherein said assay is a determination of temperature optimum for enzyme activity.

12. A method according to claim 2 wherein said assay is a determination of differential inhibition of enzyme activity.

13. A method according to claim 2 wherein said assay is a determination of differential electrophoretic mobility of the enzyme.

14. A method according to claim 1 wherein said asay is an assay of mitochondrial activity governed by the enzyme complex of an energy conserving site of the mitochondrial electron transport system of the cells.

15. A method according to claim 14 wherein said assay is a determination of calcium uptake by the mitochondria.

16. A method according to claim 14 wherein said assay is a determination of oxygen consumption by the mitochondria.

17. A method according to claim 14 wherein said assay is a determination of inhibition of oxygen consumption by the mitochondria.

10. A method for the diagnosis of cystic fibrosis, the detection of non-symptomatic carriers of the disease and identification of non-affected normal individuals, which method comprises:

(A) obtaining a sample of venous blood from a human subject,

(B) isolating the lymphocytes from said blood sample,

(C) preparing a homogenate of the lymphocyte cells,

(D) performing a kinetic assay of the NADH dehydrogenase enzyme complex of the first energy conserving site of the mitochondrial electron transport system of the lymphocyte cells by determination of the pH optimum for activity of said enzyme at pH values of about 8.0, 8.3 and 8.6, and

(E) evaluating the assay by comparison with standards established as the result of similar assays of other subjects of known conditions.