Analytical element and method for analysis of multiple analytes

• US 4,274,832 A
• Filed: 02/12/1979
• Issued: 06/23/1981
• Est. Priority Date: 02/12/1979
• Status: Expired due to Term

• Current Assignee: Clinical Diagnostic Systems Inc.
• Original Assignee: Eastman Kodak Company
• Inventors: Wu, Tai-Wing, Dappen, Glen M.
• Primary Examiner(s): Turk, Arnold
• Application Number:
  US06/011,605
• Time in Patent Office: 862 Days
• Field of Search: 23/230 B, 23/230.3, 23/230 R, 23/905, 23/909, 422/55-57, 435/11, 435/14, 435/18, 435/4, 435/805, 435/810
• US Class Current: 435/11
• CPC Class Codes: G01N 33/52 Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements immunological elements G01N33/54386; involving labelled immunochemicals G01N33/58; for haemoglobin or occult blood G01N33/72
• CPC Class Codes: Y10S 435/805 Test papers
• CPC Class Codes: Y10T 436/146666 Bile pigment

Abstract

An analytical element for the detection of two or more analytes in an aqueous liquid. The element contains an essentially dry matrix, at least a portion of the matrix containing at least two interactive compositions, a first composition generating a first radiometrically detectable species corresponding to the presence and/or concentration of one analyte, and a second composition for the inhibition or the destruction of a second radiometrically detectable species corresponding to the presence and/or concentration of one of the other analytes. The first and second interactive compositions are positioned in the element matrix to be in liquid contact with one another. Each of the first and second detectable species produced in the element is selected to have a different, characteristic, detectable absorption or emission peak in the electromagnetic spectrum.

Claims

1. An analytical element for the analysis of analytes in a liquid, said element having an essentially dry matrix permeable to said liquid, at least a portion of said matrix comprising

(a) a first interactive composition for the generation of a first radiometrically detectable species in an amount corresponding to the presence and/or concentration of one analyte, or a reaction or decomposition product thereof, and

(b) a second interactive composition for the inhibition or the destruction of a second radiometrically detectable species in an amount corresponding to the presence and/or the concentration of a second analyte, or a reaction or decomposition product thereof;

said first and second interactive compositions being in liquid contact with one another within said matrix during use, and each of said first and second radiometrically detectable species having a different characteristic, detectable absorption or emission peak.

2. An analytical element as defined in claim 1 wherein said first radiometrically detectable species is colorimetrically detectable and wherein said second radiometrically detectable species is fluorimetrically detectable, the absorption or emission peak of each of said species being in a region of the electromagnetic spectrum above 300 nm, the respective peaks being separated by at least about 5 nm.

3. An analytical element as defined in claim 1 wherein each of said first and said second radiometrically detectable species are colorimetrically detectable, the absorption peak of each of said species being in a region of the electromagnetic spectrum above 300 nm, the respective peaks being separated by at least about 5 nm.

4. An analytical element as defined in claim 1 wherein each of said first and second radiometrically detectable species are fluorometrically detectable, the emission peak of each of said species being in a region of the electromagnetic spectrum above 300 nm, the respective peaks being separated by at least about 5 nm.

5. An analytical element as defined in claim 1 wherein said first interactive composition comprises a member selected from the group consisting of

(i) an interactive mordant composition for detection of bilirubin;

(ii) a composition comprising an oxidase enzyme coupled to a hydrogen peroxide dye generating system, said composition (ii) being for the detection of an analyte representing a substrate for said oxidase enzyme;

(iii) a composition comprising an ammonia-producing enzyme coupled to a radiometric ammonia detection system which generates an increasing amount of a radiometrically detectable species in response to an increase in ammonia concentration, said composition (iii) being for the detection of an analyte representing a substrate for said ammonia-producing enzyme, and

(iv) an amylase-dyed starch enzymatic reaction composition for the detection of chloride.

6. An analytical element as defined in claim 1 wherein said second interactive composition comprises a member selected from the group consisting of

(i) a β

-glucuronidase-glucuronide enzymatic reaction composition for the detection of cholesterol;

(ii) a composition comprising an ammonia-producing enzyme coupled to a radiometric ammonia detection system which destroys an increasing amount of a radiometrically detectable species in response to an increase in ammonia concentration, said composition (ii) being for the detection of an analyte representing a substrate for said ammonia-producing enzyme; and

(iii) a bilirubin-active complex for detection of bilirubin;

said complex comprising a diffusible, bilirubin-displaceable, fluorescently detectable ligand bound to a carrier which also can bind bilirubin, said carrier having a greater binding affinity for bilirubin than for the ligand.

7. An analytical element as defined in claim 1 wherein said first interactive composition comprises an interactive mordant composition for detection of bilirubin and said second interactive composition comprises a β

-glucuronidase-glucuronide enzymatic reaction composition for the detection of cholesterol.

8. An analytical element as defined in claim 1 wherein said first interactive composition comprises an interactive mordant composition for detection of bilirubin and said second interactive composition comprises a β

-glucuronidase-glucuronide enzymatic reaction composition for the detection of cholesterol, said glucuronide of said enzymatic reaction composition for cholesterol comprising uridine diphosphate glucuronide or bromophenol blue glucuronide.

9. A multi-zone analytical element for the analysis of analytes, in liquid, said element comprising a support bearing a spreading zone and a reagent zone, said zones providing an essentially dry matrix permeable to said liquid, at least a portion of said matrix comprising

(a) a first interactive composition for the generation of a first radiometrically detectable species in an amount corresponding to the presence and/or concentration of one analyte, or a reaction or decomposition product thereof, and

(b) a second interactive composition for the inhibition or the destruction of a second radiometrically detectable species in an amount corresponding to the presence and/or the concentration of a second analyte, or a reaction or decomposition product thereof;

said first and second interactive compositions being in liquid contact with one another within said matrix during use, with at least a portion of said interactive compositions contained in said reagent zone, and each of said first and second radiometrically detectable species having a characteristic, detectable absorption or emission peak in a region of the electromagnetic spectrum above 300 nm, the respective peaks being separated by at least about 5 nm.

10. A multilayer analytical element for the analysis of analytes, in liquid, said element comprising a support bearing, in sequential order from said support, superposed registration layer, reagent layer, and spreading layer, said superposed layers providing an essentially dry matrix permeable to said liquid, at least a portion of said matrix comprising

(a) a first interactive composition for the generation of a first radiometrically detectable species in an amount corresponding to the presence and/or concentration of one analyte, or a reaction or decomposition product thereof, and

(b) a second interactive composition for the inhibition or the destruction of a second radiometrically detectable species in an amount corresponding to the presence and/or the concentration of a second analyte, or a reaction or decomposition product thereof;

said first and second interactive compositions being in liquid contact with one another within said matrix during use, with at least a portion of one of said interactive compositions contained in said reagent layer, and each of said first and second radiometrically detectable species having a characteristic, detectable absorption or emission peak in a region of the electromagnetic spectrum above 300 nm, the respective peaks being separated by at least about 5 nm.

11. An analytical element as defined in claim 10 wherein said first radiometrically detectable species is colorimetrically detectable and wherein said second radiometrically detectable species is fluorimetrically detectable.

12. An analytical element as defined in claim 10 wherein each of said first and said second radiometrically detectable species are colorimetrically detectable.

13. An analytical element as defined in claim 10 wherein said first interactive composition comprises a member selected from the group consisting of

(i) an interactive mordant composition for detection of bilirubin;

(ii) a composition comprising an oxidase enzyme coupled to a hydrogen peroxide dye generating system, said composition (ii) being for the detection of an analyte representing a substrate for said oxidase enzyme;

(iii) a composition comprising an ammonia-producing enzyme coupled to a radiometric ammonia detection system which generates an increasing amount of a radiometrically detectable species in response to an increase in ammonia concentration, said composition (iii) being for the detection of an analyte representing a substrate for said ammonia-producing enzyme; and

(iv) an amylase-dyed starch enzymatic reaction composition for the detection of chloride.

14. An analytical element as defined in claim 10 wherein said second interactive composition comprises a member selected from the group consisting of

(i) a β

-glucuronidase-glucuronide enzymatic reaction composition for the detection of cholesterol;

(ii) a composition comprising an ammonia-producing enzyme coupled to a radiometric ammonia detection system which destroys an increasing amount of a radiometrically detectable species in response to an increase in ammonia concentration, said composition (ii) being for the detection of an analyte representing a substrate for said ammonia-producing enzyme; and

(iii) a bilirubin-active complex for detection of bilirubin;

said complex comprising a diffusible, bilirubin-displaceable, fluorewcently detectable ligand bound to a carrier which also can bind bilirubin, said carrier having a greater binding affinity for bilirubin than for the ligand.

15. An analytical element as defined in claim 10 wherein said first interactive composition comprises an interactive mordant composition for detection of bilirubin and said second interactive composition comprises a β

-glucuronidase-glucuronide enzymatic reaction composition for the detection of cholesterol.

16. An analytical element as defined in claim 10 wherein said first interactive composition comprises an interactive mordant composition for detection of bilirubin and said second interactive composition comprises a β

-glucuronidase-glucuronide enzymatic reaction composition for the detection of cholesterol, said glucuronide of said enzymatic reaction composition for cholesterol comprising uridine diphosphate glucuronide or bromophenol blue glucuronide.

17. A method for the analysis of analytes in a liquid, said method comprising

(a) contacting together said liquid an an analytical element having an essentially dry matrix permeable to said liquid, at least a portion of said matrix comprising

(i) a first interactive composition for the generation of a first radiometrically detectable species in an amount corresponding to the presence and/or concentration of one analyte, or a reaction or decomposition product thereof, and

(ii) a second interactive composition for the inhibition or the destruction of a second radiometrically detectable species in an amount corresponding to the presence and/or the concentration of a second analyte, or a reaction or decomposition product thereof;

said first and second interactive compositions being in liquid contact with one another within said matrix during use, with at least a portion of one of said interactive compositions contained in said reagent layer, and each of said first and second radiometrically detectable species having a characteristic, detectable absorption or emission peak; and

(b) radimetrically detecting, after a predetermined time, a separate detectable change occurring in said element in response to the presence and/or concentration of each of said analytes, at least one detectable change being the result of said inhibition or destruction of said second radiometrically detectable species.