Apparatus for performing a radioimmunological method of determining antigens or antibodies

• US 4,276,259 A
• Filed: 08/24/1978
• Issued: 06/30/1981
• Est. Priority Date: 08/20/1976
• Status: Expired due to Term

• Current Assignee: Immuno AG Fur Chemisch-Medizinische Produkte
• Original Assignee: Immuno AG Fur Chemisch-Medizinische Produkte
• Inventors: Eibl, Johann, Aicher, Helmut
• Primary Examiner(s): Serwin, R. E.
• Application Number:
  US05/936,500
• Time in Patent Office: 1,041 Days
• Field of Search: 422/65, 422/102, 422/71, 422/104, 141/130, 73/425, 23/920, 23/230 B, 23/230.6, 424/1
• US Class Current: 422/71
• CPC Class Codes: G01N 33/537 with separation of immune complex from unbound antigen or antibody
• CPC Class Codes: Y10S 436/804 Radioisotope, e.g. radioimmunoassay
• CPC Class Codes: Y10S 436/808 Automated or kit
• CPC Class Codes: Y10S 436/809 Multifield plates or multicontainer arrays
• CPC Class Codes: Y10S 436/82 Hepatitis associated antigens and antibodies

Abstract

In an arrangement for carrying out a radioimmunological method of determining both antigens and antibodies by examining samples of human body liquids by forming an antigen/antibody-complex with a radioactively labelled antibody, a micro-titer-plate is provided with recesses for accommodating a solution of said radioactively labelled antibody and to the sample to be examined. Carriers, in the form of elongated elements secured to a holding band, are loaded with unlabelled antigen and are contacted with the sample by being inserted in the recesses of the micro-titer-plate. The carrier is washed, and the radioactivity of the carrier is measured.

Claims

1. In an arrangement for carrying out a radioimmunological method of determining antigens or antibodies of the type including a micro-titer-plate having recesses for accommodation of the samples to be examined and solid carriers loadable with a complex former, the improvement which is characterized in that the solid carriers are elongated plate-like elements and that a holding band is provided to which said elongated plate-like elements are secured, a neck piece of smaller cross section than the elongated elements is provided between the holding band and each elongated element to form pre-determined breaking points, the elongated plate-like elements are insertable in the recesses of the micro-titer-plate.

2. An arrangement as set forth in claim 1, wherein said carriers are made of plastics material.

3. An arrangement as set forth in claim 1, wherein said carrier has been loaded with antigens and antibodies by immersion in an antigen solution for between 24 and 60 hours.