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Kit for carrying out chemically induced fluorescence immunoassay

  • US 4,277,437 A
  • Filed: 12/10/1979
  • Issued: 07/07/1981
  • Est. Priority Date: 04/05/1978
  • Status: Expired due to Term
First Claim
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1. A diagnostic kit for use in a method for determining in an assay solution, the presence of an analyte in a sample suspected of containing said analyte, said analyte being a member of an immunological pair consisting of ligand and antiligand, said ligand having at least one epitopic site and said antiligand being capable of specifically binding to said epitopic site of said ligand;

  • wherein a light emitting reciprocal pair are employed as labels, said light emitting reciprocal pair consisting of a chemiluminescence source having at least one component and a quencher capable of quenching the light emitted by said chemiluminescence source without collision, said labels being conjugated to members of said immunological pair to form a chemiluminescence label conjugate and a quencher label conjugate, and wherein in said assay solution the amount of quencher brought within quenching distance of said chemiluminescence source is related to the amount of analyte in said assay medium;

    said kit comprising in relative amount to substantially optimize said method, said chemiluminescence label conjugate and said quencher labeled conjugate.

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