Sensitizers for peroxidative activity tests

• US 4,278,439 A
• Filed: 12/17/1979
• Issued: 07/14/1981
• Est. Priority Date: 12/17/1979
• Status: Expired due to Term

• Current Assignee: Miles Laboratories, Inc. (Bayer AG)
• Original Assignee: Miles Laboratories, Inc. (Bayer AG)
• Inventors: White, William I.
• Primary Examiner(s): Gron, Teddy S.
• Application Number:
  US06/104,086
• Time in Patent Office: 575 Days
• Field of Search: 23/230 B, 23/931, 23/913, 23/901, 252/408, 422/55, 422/56, 435/28
• US Class Current: 436/66
• CPC Class Codes: C12Q 1/28 involving peroxidase
• CPC Class Codes: C12Q 2326/00 Chromogens for determinations of oxidoreductase enzymes
• CPC Class Codes: G01N 33/725 using peroxidative activity
• CPC Class Codes: Y10S 436/904 Oxidation - reduction indicators

Abstract

Improved test compositions, devices and methods for the detecting of peroxidatively active substances are provided. The test compositions include an indicator capable of being oxidized in the presence of peroxidatively active substances to provide a color change, an oxidizing agent effective to oxidize said indicator, and a sensitizing agent. The sensitizing agent comprises a compound selected from the class of compounds of the general formula: ##STR1## wherein R₁ and R₂ are independently selected from hydrogen and C₁ -C₄ alkyl. Preferred compounds are benzimidazole and 5,6-dimethylbenzimidazole.

Claims

1. A test composition for detection of peroxidatively active substances in body fluids, body excreta, and the like, including an indicator capable of being oxidized in the presence of a peroxidatively active substance to provide a color change, an oxidizing agent effective to oxidize said indicator, and an effective amount of a sensitizing agent selected from the class of compounds having the formula:

##STR3## wherein R₁ and R₂ are independently selected from hydrogen and C₁ -C₄ alkyl.

2. The composition of claim 1 wherein said sensitizing agent is benzimidazole.

3. The composition of claim 1 wherein said sensitizing agent is 5,6-dimethylbenzimidazole.

4. A test device for detection of peroxidatively active substances in body fluids, body excreta, and the like, which comprises a carrier incorporated with the composition of claim 1.

5. A test device for detection of peroxidatively active substances which comprises a carrier incorporated with the composition of claim 2.

6. A test device for detection of peroxidatively active substances in body fluids, body excreta, and the like, which comprises a carrier incorporated with the composition of claim 3.

7. A method for the determination of peroxidatively active substances in body fluids, body excreta, and the like, which comprises contacting test sample with the composition of claim 1 and observing any resultant color change.