Process for treating cellulosic materials and obtaining glucose therefrom

• US 4,281,063 A
• Filed: 10/03/1979
• Issued: 07/28/1981
• Est. Priority Date: 03/08/1978
• Status: Expired due to Term

• Current Assignee: Purdue Research Foundation (The Trustees of Purdue University (d/b/a Purdue University))
• Original Assignee: Purdue Research Foundation (The Trustees of Purdue University (d/b/a Purdue University))
• Inventors: Ladisch, Michael R., Tsao, George T., Hsu, Teh-An, Ladisch, Christine M.
• Primary Examiner(s): Marantz, Sidney
• Application Number:
  US06/081,537
• Time in Patent Office: 664 Days
• Field of Search: 127/37, 435/99, 536/56
• US Class Current: 435/99
• CPC Class Codes: C12N 9/2434 acting on beta-1,4-glucosidic bonds
• CPC Class Codes: C12Y 302/01004 Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
• CPC Class Codes: C13K 1/02 obtained by saccharification of cellulosic materials manufacture of fodder A23K10/32

Abstract

A process for treating cellulosic materials to obtain glucose therefrom, which process includes an initial acid or base treatment of the cellulosic materials to remove hemicellulose, followed by a solvent treatment of the solid residue to dissolve the native cellulose contained therein. The dissolved cellulose is separated from the solid lignin-containing residue, whereafter the cellulose is reprecipitated by contacting the solution thereof with water. The reprecipitated cellulose is hydrolyzed to glucose either by acid or enzyme hydrolysis. If desired, the cellulose may be reprecipitated and hydrolyzed in the presence of the lignin-containing solid, the latter being separated from the glucose.

Claims

1. A process for providing a yield of glucose from a cellulosic material, said process comprising:

fractionating the cellulosic material into a solid cellulose-and lignin-containing fraction and a liquid fraction containing hemicellulose or C₅ sugars derived therefrom;

treating said solid cellulose- and lignin-containing fraction with a solvent for cellulose to dissolve the cellulose in said fraction and to form a lignin-containing residue;

reprecipitating the dissolved cellulose; and

hydrolyzing the reprecipitated cellulose to yield glucose.

2. The process of claim 1, wherein the dissolved cellulose is separated from said lignin-containing residue before being reprecipitated and hydrolyzed to glucose.

3. The process of claim 1, wherein the dissolved cellulose is hydrolyzed to glucose in the presence of said lignin-containing residue, whereafter said residue is separated from the glucose.

4. A process for providing a yield of glucose from a cellulosic material, said process comprising:

removing hemicellulose from the cellulosic materials;

dissolving in a solvent for cellulose the cellulosic material having said hemicellulose removed therefrom;

reprecipitating the dissolved cellulose;

hydrolyzing the reprecipitated cellulose with an enzyme or acid to yield glucose, and a lignin-containing solid residue; and

removing the lignin-containing residue from the glucose.

5. A process for providing a high yield of glucose from a cellulosic material, said process comprising:

reducing the physical size of the cellulosic material to about 5 to 100 mesh;

treating the cellulosic materials with a dilute acid or alkali to remove hemicellulose therefrom and to form a cellulose- and lignin-containing residue;

treating said residue with a solvent for cellulose to dissolve the cellulose and thereby form a liquid mixture having lignin therein in solid form,

precipitating cellulose from said liquid mixture;

hydrolyzing the precipitated cellulose by addition of acid or cellulase enzyme or a combination thereof to provide a high yield of glucose therefrom; and

separating the yielded glucose from the solid lignin.

6. A process for recovering cellulose from a cellulosic material and utilization of said recovered cellulose to provide a yield of glucose, said process comprising:

removing hemicellulose from a cellulosic material;

dissolving in a solvent for cellulose the cellulosic material having said hemicellulose removed therefrom to form a solution substantially comprised of cellulose and solvent and a lignin-containing solid residue;

separating said lignin-containing solid residue from said solution;

reprecipitating cellulose from said solution; and

hydrolyzing the reprecipitated cellulose to provide a yield of glucose.

7. The process of claim 6, wherein said cellulose is hydrolyzed by an acid and/or a cellulase enzyme to provide a high yield of glucose.

8. The process of claim 7, wherein said cellulose is hydrolyzed by use of either free or immobilized enzymes.

9. The process of claim 7, wherein said cellulase enzyme is an endo-1, 4-beta-glucanase.

10. The process of claim 9, wherein said cellulase enzyme is Tricoderma viride cellulase solution.

11. A process for providing a high yield of glucose from cellulose recovered from a cellulosic material, said process comprising:

treating a cellulosic material with a dilute acid to form a liquid fraction containing primarily C₅ sugars and a solid fraction containing primarily cellulose and lignin;

separating said liquid fraction from said solid fraction;

dissolving the cellulose in said solid fraction by contacting said fraction with a solvent, the lignin in said fraction remaining as a solid residue;

separating said solid residue from the dissolved cellulose;

reprecipitating the cellulose from said solvent; and

hydrolyzing the reprecipitated cellulose by addition of acid and/or cellulase enzyme to provide a high yield of glucose therefrom.

12. A process for providing a high yield of glucose from cellulose recovered from a cellulosic material, said process comprising:

treating a cellulosic material with a dilute base to form a liquid fraction containing primarily hemicellulose and a solid fraction containing primarily cellulose and lignin;

separating said liquid fraction from said solid fraction;

dissolving the cellulose in said solid fraction by contacting said fraction with a solvent, the lignin in said fraction remaining as a solid residue;

separating said solid residue from the dissolved cellulose;

reprecipitating the cellulose from said solvent; and

hydrolyzing the reprecipitated cellulose by addition of acid and/or cellulase enzyme to provide a high yield of glucose therefrom.

13. A process for recovering cellulose from a cellulosic material, said process comprising:

removing hemicellulose from a cellulosic material;

dissolving in a solvent for cellulose the cellulosic material having said hemicellulose removed therefrom;

separating lignin from the cellulosic material dissolved in said solvent such that a solution substantially of cellulose and solvent remains;

reprecipitating cellulose from said solution; and

recovering the reprecipitated cellulose in solid form.

14. The process of claim 13, wherein said process includes physically reducing the cellulosic material in size before removing said hemicellulose therefrom.

15. The process of claim 13, wherein said hemicellulose is removed by extraction with a dilute acid or alkali.

16. The process of claim 13, wherein the cellulosic material is dissolved in a solvent comprising from about 25-30% by weight of ethylenediamine, from about 70-75% by weight water, and from about 4.5-7% by weight of cadmium added as oxide or hydroxide.

17. The process of claim 13, wherein said solvent is recovered for reuse.