Pectin culture media and method
First Claim
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1. A method for preparing a gelled biological growth medium which comprises the steps of:
- a. providing a predetermined amount of a liquid growth medium;
b. providing a predetermined amount of a low methoxyl pectin material;
c. providing a culture-growth container having a growth capatible gel therein, the growth-compatible gel containing a multivalent metal cation material suitable to produce gelling of the low methoxyl pectin material, said growth-compatible gel containing a multivalent metal cation material comprising a layer in contact with the bottom of said culture-growth container; and
d. adding the liquid growth medium and low methoxyl pectin material to the culture growth container to produce a gelled growth medium having the low methoxyl pectin material as essentially the sole functional gelling agent.
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Abstract
A method for preparing a gelled biological growth medium which comprises combining a liquid growth medium and low methoxyl pectin material to a culture-growth container having a growth-compatible gel including a multivalent metal cation material therein, the pectin material being the sole or essentially the sole functional gelling agent of the growth medium gel.
31 Citations
34 Claims
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1. A method for preparing a gelled biological growth medium which comprises the steps of:
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a. providing a predetermined amount of a liquid growth medium; b. providing a predetermined amount of a low methoxyl pectin material; c. providing a culture-growth container having a growth capatible gel therein, the growth-compatible gel containing a multivalent metal cation material suitable to produce gelling of the low methoxyl pectin material, said growth-compatible gel containing a multivalent metal cation material comprising a layer in contact with the bottom of said culture-growth container; and d. adding the liquid growth medium and low methoxyl pectin material to the culture growth container to produce a gelled growth medium having the low methoxyl pectin material as essentially the sole functional gelling agent. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 24, 25, 26, 27, 28)
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13. A method for preparing a gelled biological growth medium which comprises the steps of:
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a. providing a predetermined amount of a liquid growth medium; b. providing a predetermined amount of a low methoxyl pectin material; c. providing a culture-growth container having located thereon a multivalent metal cation material suitable to produce gelling of the low methoxyl material, the metal cation material forming a generally evenly distributed film layer upon a surface of the culture-growth container, said film layer of multivalent metal cation material being in contact with the bottom of said culture-growth container; and d. adding the liquid growth medium and low methoxyl pectin material to the culture growth container to produce a gelled growth medium having the low methoxyl pectin material as essentially the sole functional gelling agent. - View Dependent Claims (14, 15)
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16. The combination useful as a biological growth medium which comprises:
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a culture growth container; a growth-compatible gel forming a first layer in said container, said growth-compatible gel including a material other than a pectin material as the substantial functional gelling agent and a metal cation material suitable to produce gelling of a low methoxyl pectin material, said first layer of growth-compatible gel being in contact with the bottom of said culture-growth container; and a growth-medium gel forming a second layer adjacent said growth-compatible gel in said container, said growth-medium gel including a growth medium and as essentially the sole functional gelling agent a low methoxyl pectin material. - View Dependent Claims (17, 18, 19, 20, 21, 22, 23, 29, 30)
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31. The combination useful in preparing a gelled biological growth medium comprising:
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a culture-growth container; and a growth-compatible gel located in said container said growth-compatible gel including a meterial other than a pectin material as the substantial functional gelling agent, said growth-compatible gel further including a multivalent metal cation material in an amount of at least about 4 grams/100 milliliters, said growth-compatible gel containing a multivalent metal cation material comprising a layer in contact with the bottom of said culture-growth container. - View Dependent Claims (32)
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33. The combination useful in preparing a gelled biological growth medium comprising:
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a culture-growth container; and a multivalent metal cation material forming a thin, evenly distributed film upon a surface of the culture-growth container, said film of multivalent metal cation material being in contact with the bottom of said culture-growth container. - View Dependent Claims (34)
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Specification