Apparatus and method for measuring scattering of light in particle detection systems

• US 4,286,876 A
• Filed: 01/02/1979
• Issued: 09/01/1981
• Est. Priority Date: 01/02/1979
• Status: Expired due to Term

• Current Assignee: Coulter Electronics, Inc. (Danaher Corporation)
• Original Assignee: Coulter Electronics, Inc. (Danaher Corporation)
• Inventors: Brunsting, Albert, Hogg, Walter R.
• Primary Examiner(s): Corbin, John K.
• Assistant Examiner(s): Koren, Matthew W.
• Application Number:
  US06/000,438
• Time in Patent Office: 973 Days
• Field of Search: 356/336, 356/338, 356/343, 250/574, 350/103, 350/109, 350/167, 350/211, 350/292, 350/320
• US Class Current: 356/343
• CPC Class Codes: G01N 15/0211 Investigating a scatter or diffraction pattern
• CPC Class Codes: G01N 2021/4716 Using a ring of sensors, or a combination of diaphragm and sensors; Annular sensor
• CPC Class Codes: G01N 21/47 Scattering, i.e. diffuse reflection G01N21/25, G01N21/41 take precedence G01N21/55 takes precedence

Abstract

In an apparatus in which particles are passed through an optical sensing zone to measure their light scattering characteristics for the purpose of identifying the particles, means and a method are provided for deviating the collected light in accordance with predetermined different paths to a plurality of different photodetecting devices. The deviation is effected simultaneously with collection by optical radiant energy reflecting means. The different photodetecting devices enable the measurement of energy scattered along the particular path which is identified with that device. The paths are established by the combined collecting and deviating means rather than permitted to evolve by the scattering phenomena themselves whereby the photodetecting devices can be located in convenient arrangements and may be conventional in construction.

Claims

1. Apparatus for measuring the scattering of radiant energy produced by particles for identification or the like of the said particles which comprises:

A. a source of radiant energy arranged to project a beam of radiant energy along a path,

B. a sensing zone,

C. means for moving particles through said sensing zone on an angle relative to said path to produce scattering of radiant energy from said beam,

D. combined collecting and deviating means for receiving some of the scattered radiant energy and reflecting the same selectively with respect to different geometric portions of the scattered energy to confluence points at locations laterally spaced from said first axis, said collecting and deviating means comprising

i. an assembly of spherical mirror elements having their reflective surfaces facing the sensing zone, said assembly having a central first axis passing through said sensing zone,

ii. each element being of a configuration and location to receive and collect radiant energy from a different geometric portion,

iii. each element having an optical second axis, said element being oriented with respect to the first axis such that its own optical second axis diverges from the said first axis such that it deviates the radiant energy it collects by reflection along a third reflective axis which is twice the angle between the first and second axes to a point of confluence of the deviated radiant energy that is spaced laterally from the first axis,

iv. the confluence point locations being spaced apart from one another in addition to being spaced laterally from the first axis and

E. means responsive to the intensity of radiant energy at each location.

2. The apparatus as claimed in claim 1 in which the said beam of radiant energy is coincident with said first axis.

3. The apparatus as claimed in claim 2 in which the mirror elements all derive from the same optical dimensions spherical mirror.

4. The apparatus as claimed in claim 2 in which the mirror elements are all annular.

5. The apparatus as claimed in claim 2 in which the sensing zone is located between the collecting and deviating means and the source of radiant energy whereby to enable the collecting of forward scattered radiant energy.

6. The apparatus as claimed in claim 1 in which the collecting and deviating means and the source of radiant energy are both on the same side of said sensing zone.

7. The apparatus as claimed in claim 2 in which the collecting and deviating means and the source of radiant energy are both on the same side of said sensing zone, the said assembly having a central aperture and being located between the source and sensing zone whereby to enable the collecting of back scattered radiant energy.

8. The apparatus as claimed in claim 1 in which the assembly is an integral article and the elements comprise surface formations on the face of the article.

9. The apparatus as claimed in claim 2 in which the assembly is an integral article and the elements comprise surface formations on the face of the article.

10. The apparatus as claimed in claim 1 in which the means responsive to the intensity of radiant energy comprise a plurality of independent photodetectors, one being located at least adjacent to if not at each confluence point location.

11. The apparatus as claimed in claim 2 in which the means responsive to the intensity of radiant energy comprise a plurality of independent photodetectors, one being located at least adjacent to if not at each confluence point location.

12. The apparatus as claimed in claim 10 in which there is an iris having an aperture interposed between each photodetector and its arcuate element and the confluence point individual to that element is at the aperture rather than at the photodetector.

13. The apparatus as claimed in claim 11 in which there is an iris having an aperture interposed between each photodetector and its associated element and the confluence point individual to that element is at the aperture rather than at the photodetector.

14. The apparatus as claimed in claim 12 in which means are provided to block radiant energy from direct impingement on the geometric center of each photodetector.

15. The apparatus as claimed in claim 13 in which means are provided to block radiant energy from direct impingement on the geometric center of each photodetector.

16. The apparatus as claimed in claim 12 which includes optical means between each aperture and its associated photodetector for additionally focussing the radiant energy onto its said photodetector.

17. The Apparatus as claimed in claim 13 which includes optical means between each aperture and its associated photodetector for additionally focussing the radiant energy onto its said photodetector.

18. A composite mirror of multiple reflecting surface elements adapted to receive distributed radiant energy from a source that is directing such radiant energy in a conical solid angle generally along a principal optical axis directed toward the composite mirror, said mirror adapted to reflect at least portions and at most all of the radiant energy it receives back towards said source generally but along reflective axes which diverge from said principal optical axis so that different surface elements will receive different geometric zones of the distributed radiant energy and reflect and cause confluence of the energy in the respective zones at different locations laterally of the principal optical axis where the energy can be measured, said surface elements each being derived from and comprising a portion of a spherical mirror whose individual optical axis is tilted by a first angle relative to said principal optical axis whereby its reflective axis diverges from said principal optical axis at an angle which is twice said first angle.

19. The composite mirror as claimed in claim 18 in which the surface elements are joined in a single integral structure.

20. The composite mirror as claimed in claim 18 in which all of the surface elements are all derived from the same curvature spherical mirror.

21. The composite mirror as claimed in claim 18 in which the elements are all annular and of different diameters.

22. The composite mirror as claimed in claim 18 in which at least some of the elements have different first angles of tilt.

23. The composite mirror as claimed in claim 18 in which all of the elements have first angles of tilt differing from one another whereby all of the points of confluence are spaced differently from the principal optical axis.

24. The composite mirror as claimed in claim 21 in which the center element is circular and has a central aperture for passage of a radiant energy beam to the source.

25. A method of measuring the directional distribution properties of a particle for particle identification or the like which comprises:

A. passing the particle through a sensing zone and illuminating the particle with an incident beam of radiant energy whereby to produce reactive directional distribution of the radiant energy as a result of the intersection of particle and beam,

B. intercepting a portion of the radiant energy which is directionally distributed and which is projected to the location of interception in a conical solid angle whose approximate principal optical axis extends between the point of intersection and the location of interception,

C. reflecting the intercepted radiant energy along a plurality of different paths which diverge from the principal axis and confluence at locations laterally of the axis,

i. each path being arranged to comprise the radiant energy intercepted from an arcuate solid angle of the projected portion,

ii. the arcuate areas being different from one another,

iii. the axis of each path being a reflective axis

iv. the locations of confluence being spaced from one another, and

D. measuring the respective intensities of the radiant energy at the locations of confluence.

26. The method as claimed in claim 25 in which the properties measured are the scattering properties of the particle and the intersection of the particle and beam causes scattering.

27. The method as claimed in claim 26 in which the arcuate solid angles of the projected portion comprise cones of radiant energy and each path is individual to a different cone, the cones being of increasing size from the center principal axis radially outward and encompassing substantially all of the intercepted radiant energy, the number of paths being equal to the number of cones.

28. The method as claimed in claim 26 in which the particle is illuminated from a source which is also directed toward the location of interception whereby the radiant energy measured comprises forward scattered radiant energy.

29. The method as claimed in claim 26 in which the particle is illuminated from a source that is directed through the location of interception whereby the radiant energy measured comprises back scattered radiant energy.

30. The method as claimed in claim 27 wherein the reflecting is effected in the manner of reflection from spherical mirror elements.