Method and apparatus for measuring microorganism activity

• US 4,288,544 A
• Filed: 05/01/1979
• Issued: 09/08/1981
• Est. Priority Date: 08/23/1978
• Status: Expired due to Term

• Current Assignee: Kyowa Hakko Kogyo Company Limited (Kirin Holdings Company Limited)
• Original Assignee: Kyowa Hakko Kogyo Company Limited (Kirin Holdings Company Limited)
• Inventors: Suzuki, Shuichi, Karube, Isao
• Primary Examiner(s): Warden, Robert J.
• Application Number:
  US06/035,092
• Time in Patent Office: 861 Days
• Field of Search: 435/4, 435/29, 435/32, 435/34, 435/36, 435/37, 435/38, 435/39, 435/40, 435/291, 435/287, 435/289, 435/817, 204/1 E, 204/1 T, 204/195 R, 204/195 B
• US Class Current: 205/777.5
• CPC Class Codes: C12Q 1/04 Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor C12Q1/6897 takes precedence
• CPC Class Codes: C12Q 2304/80 Electrochemical detection via electrodes in contact with culture medium

Abstract

The microbial activity in a liquid medium is determined by measuring the electric current values or potential values generated by the microorganisms. Also disclosed herein is apparatus for carrying out such measurement comprising two electrodes or two electrolytic electrode systems constructed and arranged to be immersed in a culture liquor.

Claims

1. A method for determining the unknown number of living cells or total number of active parts in a liquid medium by measuring the difference between the current value or potential value of said microorganism and the current value or potential value of said liquid medium and correlating said measured difference with a current or potential difference which corresponds to a known amount of said microorganism.

2. A method according to claim 1 wherein said measurement is carried out by suspending in said liquid medium two electrode systems, each having a cathode, an internal electrolyte and a liquid junction and each having an exposed anode, one of said anodes being covered with a microorganism-impermeable membrane;

and comparing the current value or potential value of said two electrode systems.

3. A method according to claim 1 wherein said measurement is carried out by suspending in said liquid medium two electrolytic electrode potentiostatic systems comprising two exposed electrodes and a reference calomel electrode, one of said electrolytic electrode systems having one of said exposed electrode (anode) being covered with a microorganism-impermeable membrane, and by comparing the current value or potential value of said two electrolytic electrode systems.

4. Apparatus for measuring microorganism activity in a liquid medium which comprises two electrode systems each of which comprises a cathode with an inside electrolyte having a liquid junction with said liquid medium and an exposed anode, wherein the exposed anode of one of said systems is covered with a microorganism-impermeable membrane for measuring activity of the microorganism on the outer side of said membrane;

each of said cathodes and anodes being arranged in parallel and being connected to an ammeter or potentiometer.