Process for the large scale production of pituitary hormones by serial secondary suspension culture
First Claim
1. A nutrient culture medium composition adapted to the production of Prolactin from the pituitary by serial sub-culturing, said composition consisting essentially of amino acid-rich nutrient Medium 199-1X with Earle'"'"'s modified salts supplemented by the addition of about 5 to 20 ml of liver extract and about 6 to 20 I.U. of insulin per liter of Medium 199-1X, minor amounts of anti-bacterial and anti-fungal agents and about 10 to 100 mg per liter of Medium 199-1X of an aqueous suspension of a substance with progesteron-androgenic activity.
1 Assignment
0 Petitions
Accused Products
Abstract
A new system of serially culturing anterior pituitary gland cells in a new nutrient medium under open aeration to produce large amounts of pituitary hormones. Using the new system of culture and new nutrient media, the pituitary cells can be grown in vitro to produce in approximately three weeks 10 to 12 times the amount of extractable hormone originally present in the original tissue.
-
Citations
4 Claims
- 1. A nutrient culture medium composition adapted to the production of Prolactin from the pituitary by serial sub-culturing, said composition consisting essentially of amino acid-rich nutrient Medium 199-1X with Earle'"'"'s modified salts supplemented by the addition of about 5 to 20 ml of liver extract and about 6 to 20 I.U. of insulin per liter of Medium 199-1X, minor amounts of anti-bacterial and anti-fungal agents and about 10 to 100 mg per liter of Medium 199-1X of an aqueous suspension of a substance with progesteron-androgenic activity.
Specification