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Process for the large scale production of pituitary hormones by serial secondary suspension culture

  • US 4,288,546 A
  • Filed: 11/06/1978
  • Issued: 09/08/1981
  • Est. Priority Date: 04/09/1976
  • Status: Expired due to Term
First Claim
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1. A nutrient culture medium composition adapted to the production of Prolactin from the pituitary by serial sub-culturing, said composition consisting essentially of amino acid-rich nutrient Medium 199-1X with Earle'"'"'s modified salts supplemented by the addition of about 5 to 20 ml of liver extract and about 6 to 20 I.U. of insulin per liter of Medium 199-1X, minor amounts of anti-bacterial and anti-fungal agents and about 10 to 100 mg per liter of Medium 199-1X of an aqueous suspension of a substance with progesteron-androgenic activity.

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