Radioimmunoassay plate

• US 4,292,273 A
• Filed: 06/29/1979
• Issued: 09/29/1981
• Est. Priority Date: 06/29/1979
• Status: Expired due to Term

• Current Assignee: Data Packaging Corporation
• Original Assignee: Data Packaging Corporation
• Inventors: Butz, David E., Pepicelli, Pasquale L.
• Primary Examiner(s): Castel, Benoit
• Application Number:
  US06/053,260
• Time in Patent Office: 823 Days
• Field of Search: 435/301, 435/287, 435/291, 435/298, 435/300, 435/810, 422/102, 422/104, 422/61, 422/99, 206/564, 220/23.6, 220/23.8
• US Class Current: 435/288.4
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2300/0851 Bottom walls
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: B01L 3/50853 with covers or lids
• CPC Class Codes: C12M 23/12 Well or multiwell plates C12M25/04 takes precedence
• CPC Class Codes: C12M 23/22 Transparent or translucent parts glassware for laboratory use B01L3/00
• CPC Class Codes: C12M 23/38 Caps; Covers; Plugs; Pouring means
• CPC Class Codes: C12M 23/46 Means for fastening

Abstract

A radioimmunoassay plate composed of a tray and a cover. The tray has a plurality of wells having downwardly tapered side walls and coplanar bottom walls. Each bottom wall has a flat central portion and rounded peripheral portion, the peripheral portion merging into the side wall.

Claims

1. A radioimmunoassay plate comprising:

a bottom tray made of transparent material and having a plurality of wells extending downwardly from its top plane with the wells defined by downwardly tapered side walls and a bottom wall, said bottom walls lying in a common plane parallel to the top plane of the tray,

a peripheral side wall extending downwardly from the top plane and terminating at its lower edge above the plane of the bottom walls, whereby the bottom walls may all contact a planar surface on which the tray is placed without interference from the peripheral side walls,

each of said well bottom walls being flat at its central portion and having a substantial radius at the junction of the bottom and side wall equal to approximately half the radius of the bottom wall,

a removable cover for the bottom tray having a top wall which is adapted to closely overlie the top plane of the tray and having a skirt which engages and is supported by the peripheral side wall of the tray and forms a seal therewith when positioned to cover the tray and the wells in it,

an outwardly extending flange formed at the bottom of the peripheral side wall of the tray,

a seat formed in the cover for receiving the flange of a second bottom tray stacked on it to support the second tray on the cover,

and means provided in the cover for preventing a second bottom tray stacked on it from sliding sideways off the cover.

2. A radioimmunoassay plate as defined in claim 1 further characterized by

an outwardly extending flange formed at the bottom of the skirt of the cover,

said side wall and skirt forming a surface to surface seal with the flanges separated when the cover is in place on the tray, said flanges facilitating removal of the cover from the tray.

3. A radioimmunoassay plate comprising:

a tray having a plurality of wells for receiving material and each defined by a downwardly extending side wall and a bottom wall;

said bottom walls having coplanar flat, central portions and rounded peripheral portions which merge smoothly into the side walls for preventing the material from forming a halo image on film exposed against the bottom walls while enabling all the bottom walls to make surface to surface contact on a planar sheet of film on which the tray is placed,

said tray having a peripheral side wall with the lowermost portion of the side wall disposed above the flat central portions of the bottom walls of the wells so as not to interfere with said contact of the flat central portions with the sheet of film,

the flat central portion of each of the well bottom walls being approximately half the diameter of the well measured to the side wall,

a cover removably mounted on the tray and having a top wall that overlies the top of the wells when the cover is mounted on the tray,

said cover having a skirt extending downwardly from the top wall for engaging and forming a seal with the tray to close the wells to ambient atmosphere,

and means provided in the cover for supporting a second tray stacked on it identical to the tray covered by it.

4. A radioimmunoassay plate as defined in claim 3 further characterized by

said means in the cover including a shoulder at the periphery of the cover for receiving the bottom edge of the peripheral side wall of the second tray, a bead formed in the cover inwardly of the shoulder to engage the inside surface of the peripheral side wall to prevent the second tray from moving laterally on the cover, and a recess in the top wall radially inside the bead and in a place spaced below the plane of the shoulder so as not to engage the bottoms of the wells of the second tray and interfere with the seating of the peripheral wall of the second tray on said shoulder.

5. A radioimmunoassay plate as defined in claim 3 further characterized by

said well side walls tapering in a downwardly direction.

6. A radioimmunoassay plate as defined in claim 3 further characterized by

said well side walls being circular in cross section.

7. A radioimmunoassay plate as defined in claim 6 further characterized by

said cover skirt engaging the peripheral side wall of the tray to form the seal with the tray.

8. A radioimmunoassay plate as defined in claim 3 wherein the tray is transparent.