Magnetic attraction transfer devices for use in solid phase radioimmunoassays and in other assay methods

• US 4,292,920 A
• Filed: 12/20/1979
• Issued: 10/06/1981
• Est. Priority Date: 04/26/1976
• Status: Expired due to Term

• Current Assignee: Kendall O. Smith, Warren D. Gehle
• Original Assignee: Kendall O. Smith, Warren D. Gehle
• Inventors: Gehle, Warren D., Smith, Kendall O.
• Primary Examiner(s): Pianalto, Bernard D.
• Application Number:
  US06/105,707
• Time in Patent Office: 656 Days
• Field of Search: 118/400, 118/425, 118/500
• US Class Current: 118/425
• CPC Class Codes: B03C 1/0332 using permanent magnets
• CPC Class Codes: G01N 33/54326 Magnetic particles
• CPC Class Codes: G01N 35/0098 involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C

Abstract

A device for transferring, by magnetic attraction, antigen-antibody adsorbent materials from one reaction mixture to another, and to facilitate an efficient rinsing of these materials, and for transferring adsorbent materials used in other assay methods.

Claims

1. A device for use in solid phase immumoassays, for simultaneously transferring a plurality of antigen-antibody absorbent units from one location to another, comprising a transfer element in combination with a tray of non-magnetic material having a plurality of spaced receptacles therein for containing an appropriate liquid and for containing units each comprising a core of ferromagnetic material, each said core being covered with at least one coating for preventing chemical interactions between said core and aqueous chemical solutions and for absorbing antigens or antibodies on the outer surface thereof, said transfer element having a first portion of ferromagnetic material overlying said spaced receptacles in a transfer condition of said element, and said element having a second portion of non-magnetic material overlying spaces between said receptacles when in said transfer condition, and one of said ferromagnetic core and transfer device materials having magnetic properties to effect a magnetic attraction between said units and said transfer device, whereby said units are removable from said receptacles by magnetic attraction while being maintained in spaced apart relationship by said non-magnetic material during said transfer condition.

2. The device according to claim 1, wherein cooperating means are provided on said tray and on said transfer element for effecting said aligned condition therebetween.

3. The device according to claim 1, wherein said ferromagnetic material of said transfer element comprise a plurality of ferromagnetic elements spatially oriented relative to said spaced receptacles.

4. The device according to claims 1, 2 or 3, wherein each said unit has an inner coating for preventing chemical interactions between said core and the aqueous chemical solutions and an exterior coating for adsorbing antigens or antibodies on said outer surface thereof.

5. The device according to claim 4, wherein said exterior coating comprises an adsorbing coat selected from the group consisting of polycarbonate, polyethylene, polystyrene, polypropylene, acrylics, glass and chromatographic substances.

6. The device according to claim 4, wherein said inner coating includes a stable metal selected from the group consisting of nickel, gold, zinc, chromium, cadmium and copper, for effectively sealing said core from penetration and chemical interaction with aqueous chemical solutions.

7. The device according to claim 6, wherein said exterior coating comprises an adsorbing coat selected from the group consisting of polycarbonate, polyethylene, polystyrene, polypropylene, acrylics, glass and chromatographic substances.

8. The device according to claim 4, wherein said inner coating comprises waterproof paint material for effectively sealing said core from penetration and chemical interaction with the aqueous chemical solutions.

9. The device according to claim 8, wherein said exterior comprises an adsorbing coat selected from the group consisting of polycarbonate, polyethylene polystyrene, polypropylene, acrylics, glass and chromatographic substances.

10. The device according to claims 1, 2, or 3, wherein said transfer element is at least partially coated with a material capable of reducing or preventing adsorption of antibodies or antigens to its surface and of preventing degradation of said non-magnetic material of said transfer element during exposure to aqueous chemical solutions used in solid phase immunoassays.

11. The device according to claim 3, wherein said ferromagnetic elements are disposed flush with a surface of said transfer element.

12. The device according to claim 3, wherein said ferromagnetic elements extend outwardly of a surface of said transfer element, and a release member of non-magnetic material surrounding said ferromagnetic elements is provided for releasing said units adhering thereto by movement of said release member away from said surface.