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Method for determining ammonia and sensor therefor

  • US 4,297,173 A
  • Filed: 05/22/1980
  • Issued: 10/27/1981
  • Est. Priority Date: 05/22/1980
  • Status: Expired due to Term
First Claim
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1. A method for determining ammonia concentration in an aqueous liquid which contains dissolved oxygen, which comprises:

  • (a) Contacting a sample of said liquid with a microbial electrode comprising;

    an oxygen-sensitive electrode having a diaphragm,bacteria capable of oxidizing ammonium ion and thereby consuming oxygen in direct or close contact with the diaphragm of said oxygen sensitive electrode, said bacteria being confined or immobilized by a porous membrane permeable to ammonia and to oxygen and impermeable to microbial cells; and

    (b) electrochemically sensing the rate of consumption or their decrease in steady state concentration of said oxygen caused by said microorganisms, and thereby(c) detecting the concentration of ammonia from said rate or said decrease of the oxygen.

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