Hemolysing solution preparing hemolysed blood having a stabilized glucose content

• US 4,297,238 A
• Filed: 11/21/1979
• Issued: 10/27/1981
• Est. Priority Date: 11/22/1978
• Status: Expired due to Term

• Current Assignee: Merck Patent GmbH (Merck & Co., Inc.)
• Original Assignee: Merck Patent GmbH (Merck & Co., Inc.)
• Inventors: Vormbrock, Rolf, Helger, Roland
• Primary Examiner(s): Gron, Teddy S.
• Application Number:
  US06/096,347
• Time in Patent Office: 706 Days
• Field of Search: 252/408, 23/230 B, 23/901, 23/913, 435/4, 435/14, 424/2, 424/3, 424/7
• US Class Current: 436/17
• CPC Class Codes: C12Q 1/54 involving glucose or galactose
• CPC Class Codes: G01N 33/66 involving blood sugars, e.g. galactose
• CPC Class Codes: Y10T 436/107497 Preparation composition [e.g., lysing or precipitation, etc.]
• CPC Class Codes: Y10T 436/144444 Glucose
• CPC Class Codes: Y10T 436/2525 Stabilizing or preserving

Abstract

A hemolysing solution for use in the determination of glucose in a whole blood sample which comprises (a) a buffer providing a pH of about 6-8, (b) sodium chloride, (c) a surface active agent and, (d) a chelating agent which in the presence of component (c) serves as a stabilizer against glycolysis in the hemolysate obtained by contacting the blood sample with said hemolysing solution.

Claims

1. In a hemolysing solution used in the determination of glucose in a whole blood sample, consisting essentially of a lysing reagent and a glycolysis inhibitor which inhibits glycolysis in the hemolysate obtained by contacting the blood sample with the hemolysing solution, the improvement wherein the hemolysing solution consists essentially of

(a) a buffer providing a pH of about 6-8;

(b) 0.8-3.0 moles/liter of sodium chloride;

(c) 1-4 g/l of a surface active agent;

(d) 0.5-2 g/l of a chelating agent which in the presence of component (c) serves as a stabilizer against glycolysis in the hemolysate; and

, optionally,

(e) an effective amount of an antimicrobial agent.

2. A hemolysing solution of claim 1, wherein the surface-active agent is a non-ionic surface-active agent.

3. A hemolysing solution of claim 2, wherein the surface-active agent is an alkylphenol polyglycol ether.

4. A hemolysing solution of claim 1, wherein the chelating agent is ethylenediaminetetraacetate, nitrilotriacetate, cyclohexylene-1,2-dinitrilotetraacetate, diethylenetriaminepentaacetate or bis-(aminoethyl)-glycol ether-N,N,N'"'"',N'"'"'-tetraacetate.

5. A hemolysing solution of claim 1 additionally comprising sodium azide as an antimicrobial agent.

6. A method for hemolysing whole blood and for stabilizing the concentration of glucose in the hemolysate, comprising treating whole blood with the aqueous hemolysing solution of claim 1.

7. The hemolysing solution of claim 1, wherein the surface active agent is an alkylphenol polyglycol ether and the chelating agent is ethylenediaminetetraacetate, nitrilotriacetate, cyclohexylene-1,2-dinitrilotetraacetate, diethylenetriaminepentaacetate or bis-(aminoethyl)-glycol ether-N,N,N'"'"',N'"'"'-tetraacetate.