Hemolysing solution preparing hemolysed blood having a stabilized glucose content
First Claim
1. In a hemolysing solution used in the determination of glucose in a whole blood sample, consisting essentially of a lysing reagent and a glycolysis inhibitor which inhibits glycolysis in the hemolysate obtained by contacting the blood sample with the hemolysing solution, the improvement wherein the hemolysing solution consists essentially of(a) a buffer providing a pH of about 6-8;
- (b) 0.8-3.0 moles/liter of sodium chloride;
(c) 1-4 g/l of a surface active agent;
(d) 0.5-2 g/l of a chelating agent which in the presence of component (c) serves as a stabilizer against glycolysis in the hemolysate; and
, optionally,(e) an effective amount of an antimicrobial agent.
1 Assignment
0 Petitions
Accused Products
Abstract
A hemolysing solution for use in the determination of glucose in a whole blood sample which comprises (a) a buffer providing a pH of about 6-8, (b) sodium chloride, (c) a surface active agent and, (d) a chelating agent which in the presence of component (c) serves as a stabilizer against glycolysis in the hemolysate obtained by contacting the blood sample with said hemolysing solution.
-
Citations
7 Claims
-
1. In a hemolysing solution used in the determination of glucose in a whole blood sample, consisting essentially of a lysing reagent and a glycolysis inhibitor which inhibits glycolysis in the hemolysate obtained by contacting the blood sample with the hemolysing solution, the improvement wherein the hemolysing solution consists essentially of
(a) a buffer providing a pH of about 6-8; -
(b) 0.8-3.0 moles/liter of sodium chloride; (c) 1-4 g/l of a surface active agent; (d) 0.5-2 g/l of a chelating agent which in the presence of component (c) serves as a stabilizer against glycolysis in the hemolysate; and
, optionally,(e) an effective amount of an antimicrobial agent. - View Dependent Claims (2, 3, 4, 5, 6, 7)
-
Specification