Biological receptacle

• US 4,299,920 A
• Filed: 01/14/1980
• Issued: 11/10/1981
• Est. Priority Date: 01/19/1979
• Status: Expired due to Term

• Current Assignee: Peters J. Hinrich
• Original Assignee: Peters J. Hinrich
• Inventors: Peters, J. Hinrich
• Primary Examiner(s): Warden, Robert J.
• Application Number:
  US06/111,759
• Time in Patent Office: 666 Days
• Field of Search: 435/284, 435/285, 435/286, 435/240, 435/241, 435/297, 435/298, 435/299, 435/300, 435/301
• US Class Current: 435/288.4
• CPC Class Codes: C12M 23/12 Well or multiwell plates C12M25/04 takes precedence

Abstract

A receptacle for cell cultures or biological tests comprising a base plate, and a wall member joined in detachable and liquid-tight manner to the base plate, the base plate and wall defining at least one chamber, at least that portion of the wall adjacent the base plate consisting of a noncytotoxic elastomeric synthetic material adhering to the base plate.

Claims

1. A receptacle containing a test medium such as a cell culture or biological specimen consisting of a base plate and wall member, the wall member having a flat smooth bottom self-adhering to the base plate in liquid-tight manner, the wall member being formed of noncytotoxic hydrophobic elastomeric synthetic material compatible with the test medium and defining with the base plate a plurality of independent chambers, and a plurality of test media in the independent chambers.

2. A receptacle according to claim 1, wherein the synthetic material is a silicone rubber.

3. A receptacle according to claim 1, wherein the chamber tapers toward the base plate.

4. A receptacle according to claim 1, wherein the height of the chamber ranges from 0.1 to 20 mm.

5. A receptacle according to claim 1, wherein the base plate is made of a plastic material compatible with cells.

6. A receptacle according to claim 1, wherein the base plate includes pores permitting the passage therethrough of molecules, particles, cells, or cell projections.

7. A receptacle according to claim 1, including a loose-fitting cover, having an overlapping edge.

8. A receptacle according to claim 1, including an adhesive cover.

9. A receptacle according to claim 1, wherein the wall member has an adhesive surface which has a satin finish and/or is interrupted by groove- or cup-like depressions.

10. A receptacle according to claim 1, wherein test substances are applied to the base plate in a pattern corresponding to the grid of the chambers of the wall member.

11. A receptacle according to claim 1, in combination with a frame permitting the assembly of wall and base plate in alignment with one another.