Electrode-containing device with capillary transport between electrodes

• US 4,302,313 A
• Filed: 03/10/1980
• Issued: 11/24/1981
• Est. Priority Date: 07/23/1979
• Status: Expired due to Term

• Current Assignee: Clinical Diagnostic Systems Inc.
• Original Assignee: Eastman Kodak Company
• Inventors: Columbus, Richard L.
• Primary Examiner(s): Turk, Arnold
• Application Number:
  US06/128,413
• Time in Patent Office: 624 Days
• Field of Search: 422/55-58, 422/68, 422/99, 422/100, 435/4, 435/310, 23/230 R, 356/244, 204/195 R, 204/195 M, 204/195 F
• US Class Current: 204/409
• CPC Class Codes: B01L 2300/069 Absorbents; Gels to retain a fluid
• CPC Class Codes: B01L 2300/0825 Test strips
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2300/0874 Three dimensional network
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2300/089 Virtual walls for guiding liquids
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/0688 surface tension valves, capillary stop, capillary break
• CPC Class Codes: B01L 3/5023 with a sample being transported to, and subsequently stored in an absorbent for analysis
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: G01N 27/307 Disposable laminated or multilayered electrodes G01N27/3272 takes precedence

Abstract

A device suitable for potentiometric analysis of liquid ions is disclosed, wherein a liquid transport member extending between and adjacent to two electrodes comprises two opposed surfaces spaced apart a distance to induce capillary flow of liquid between the surfaces.

Claims

1. In a device for determining the activity of an ionic analyte of a liquid, said device including a pair of solid electrodes each constructed to generate an electrical potential that is proportional to the activity of an ionic analyte of a contacting quantity of such a liquid, and flow control means for directing flow of respective quantities of two such liquids each to a different one of said electrodes and to a junction between said liquids;

the improvement wherein said flow control means includes

(a) two opposed liquid transport surfaces extending from a first location disposed adjacent one of said electrodes to a second location adjacent the other of said electrodes, said surfaces being spaced apart a distance effective to induce capillary flow between said surfaces of introduced liquid and to create a transport zone, said transport zone being in liquid communication with said electrodes and comprising a void zone with fibers in an amount that is from 0 to 5% of the total volume of said zone; and

(b) means defining first and second liquid passageways extending from an exterior surface of said device to said zone;

whereby each of said liquids, when deposited in a different one of said passageways, respectively flows into contact with an electrode and through said transport zone to form a junction with the other liquid intermediate said first and second locations.

2. A device as defined in claim 1, wherein at least one of said transport surfaces is generally smooth.

3. A device as defined in claim 1, and further including, electrically isolated from said electrode pair, a second pair of solid electrodes constructed and adapted for the quantitative detection of an analyte different from the analyte detectable by said electrode pair.

4. A device as defined in claim 3, wherein both an electrode from said electrode pair and from said second pair of solid electrodes are adjacent to said first passageway, and both of the other electrodes from said electrode pair and from said second pair are adjacent to said second passageway.

6. A device as defined in claim 1 or 5, wherein at least said transport surfaces are formed from a non-fibrous material.

7. A device as defined in claim 1 or 5, wherein said zone is substantially free of fibers.

8. A device as defined in claim 1 or 5, wherein each of said passageways has an axis that is generally perpendicular to said fibers.

9. A device as defined in claim 1 or 5, and further including means defining a third liquid flow passageway extending from said exterior surface to said zone, said third passageway being approximately centered between said first and second locations, whereby an additional liquid can be added to said zone.

10. A device a defined in claim 1 or 5, wherein said transport surfaces each include, across at least portions thereof, a plurality of exposed grooves formed in a predetermined pattern, at least a substantial portion of said grooves of one of said transport surfaces being disposed at a positive angle with respect to the directly opposed grooves of the other transport surface to direct the liquid introduced between said surfaces to advance by capillary attraction along predetermined flow paths and with a predetermined peripheral wave front configuration.

11. A device as defined in claim 10, wherein the grooves of each surface are substantially linear and parallel and said angles are about 90°

.

12. A device as defined in claim 1 or 5, wherein said liquid passageways comprise a first pair of apertures in one of said surfaces, and further including a second pair of apertures in the other of said surfaces, each of said second apertures being aligned with a different one of said first pair of apertures.

13. A device as defined in claim 12, wherein each of said first pair of apertures includes aperture wall means configured for driving a quantity of liquid from an edge of the aperture into the central portion of the aperture.

14. A device as defined in claim 13, wherein each of said first pair of apertures is a hexagon.

15. A device as defined in claim 1 or 5, and further including means in said device for venting air from said transport zone to the atmosphere exterior of said device as said zone fills with liquid.

16. A device as defined in claim 15, wherein said venting means comprises a vent channel connected to said transport zone by means for inhibiting movement of said liquids into said vent channel.

17. A device as defined in claim 16, wherein said inhibiting means comprises an edge of intersection formed by a wall of said vent channel intersecting one of said transport surfaces.

5. In a device for determining the activity of an ionic analyte of a liquid, said device including a pair of solid, generally flat electrodes each constructed to generate an electrical potential that is proportional to the activity of an ionic analyte of a contacting quantity of such a liquid, and flow control means for directing flow of respective quantities of two such liquids each to a different one of said electrodes and to a junction between said liquids;

the improvement wherein said flow control means includes

(a) two opposed liquid transport surfaces extending from a first location disposed adjacent one of said electrodes to a second location adjacent the other of said electrodes, said surfaces being spaced apart a distance effective to induce capillary flow between said surfaces of introduced liquid and to create a transport zone, said transport zone being in liquid communication with said electrodes and comprising a void zone with fibers in an amount that is from 0 to 5% of the total volume of said zone; and

(b) means defining first and second liquid passageways extending from an exterior surface of said device to said zone;

whereby each of said liquids, when deposited in a different one of said passageways, respectively flows into contact with an electrode and through said transport zone to form a junction with the other liquid intermediate said first and second locations.

18. In a device for determining the activity of an ionic analyte of a liquid, said device including a pair of solid electrodes each constructed to generate an electrical potential that is proportional to the activity of an ionic analyte of a contacting quantity of such a liquid, and flow control means for directing flow of such liquid to at least one of said electrodes;

the improvement wherein said flow control means includes

(a) two opposed liquid transport surfaces extending from a first location disposed adjacent one of said electrodes to a second location adjacent the other of said electrodes, said surfaces being spaced apart a distance effective to induce capillary flow between said surfaces of introduced liquid and to create a transport zone, said transport zone being in liquid communication with said electrodes and comprising a volume containing fibers in an amount no greater than 5% of the total volume of said zone; and

(b) means defining a liquid passageway extending from an exterior surface of said device to said zone;

whereby a quantity of such liquid deposited in said passageway flows into contact with an electrode.

19. A device as defined in claim 18, and further including means in said device for venting air from said transport zone to the atmosphere exterior of said device as said zone fills with liquid.

20. A multi-analyte test device for the analysis of a plurality of different ionic analytes in a quantity of a liquid, the device comprising,

a first sheet,

a second covering sheet, said sheets having opposing surfaces,

means for spacing apart said sheets a distance effective to induce capillary flow between said surfaces of introduced liquid and to create a transport zone, said zone further including fibers in an amount that is from 0 to 5% of the total volume of said zone, one of said sheets including means permitting introduction of a quantity of a test liquid and a reference liquid into said zone, and

a plurality of electrically isolated electrodes disposed in liquid communication with said zone, at least a first one of said electrodes comprising a test composition specific to the analysis of one of said analytes, and at least a second of said electrodes comprising a test composition specific to the analysis of a different one of said analytes, and

vent means for permitting air to exit from said zone ahead of advancing liquid.

21. A device as defined in claim 20, wherein said electrodes are ion-selective electrodes.

22. A device as defined in claim 20, wherein another of said electrodes is identical to said first electrode and another of said electrodes is identical to said second electrode, whereby the analysis of the different analytes proceeds differentially.

23. A device as defined in claim 20, and further including a general reference electrode disposed in liquid communication with said zone.

24. A device as defined in claim 20, 22 or 23, wherein at least a portion of at least one of said spaced-apart sheets is provided with a plurality of exposed grooves.

25. A test device as defined in claim 20, 22 or 23, wherein said electrodes are generally flat.