Method and apparatus for making a rapid measurement of the hematocrit of blood

• US 4,303,336 A
• Filed: 08/28/1978
• Issued: 12/01/1981
• Est. Priority Date: 08/28/1978
• Status: Expired due to Term

• Current Assignee: Baxter Travenol Laboratories Incorporated (Baxter International Inc.)
• Original Assignee: Baxter Travenol Laboratories Incorporated (Baxter International Inc.)
• Inventors: Cullis, Herbert M.
• Primary Examiner(s): McGraw, Vincent P.
• Assistant Examiner(s): Bovernick, Rodney B.
• Application Number:
  US05/937,089
• Time in Patent Office: 1,191 Days
• Field of Search: 356/39, 356/432, 356/441, 356/442, 356/41, 250/576, 128/633, 128/634, 128/666
• US Class Current: 356/39
• CPC Class Codes: A61B 5/14535 for measuring haematocrit
• CPC Class Codes: G01N 21/59 Transmissivity G01N21/25 takes precedence

Abstract

The method includes the steps of: placing blood in a transparent tubing; passing intense, coherent light through the tubing and blood; sensing the light passed through the tubing and blood; and, producing a signal related to the amount of transmitted light sensed and indicative of the hematocrit of the blood. The apparatus includes a laser light source for generating the intense, coherent light beam at a wavelength in the red spectrum and an optical photodetector including a silicon diode positioned to receive the transmitted light and an amplifier circuit.

Claims

1. Method for making a non-invasive, non-destructive, rapid, relatively accurate measurement in vivo of the hematocrit of undiluted blood including the steps of:

passing the undiluted blood through a liquid confining means having a light transmitting portion;

passing intense, coherent, monochromatic light through the light transmitting portion and undiluted blood;

sensing the transmittance of light passed through the light transmitting portion and undiluted blood;

producing an output signal related to the amount of transmitted light sensed and indicative of the light absorbed by the red blood cells; and

multiplying the output signal by a proportionality factor determined from a curve function obtained from an approximated curve fit of absorbance v. hematocrit measurements for measurements of light absorbance by blood having known hematocrits to produce a corrected output signal which is indicative of the hematocrit of the undiluted blood and which can be used to control the flow rate of at least one pump pumping whole blood or a fluid component thereof in a blood processing apparatus.

2. The method according to claim 1 wherein said liquid confining means is a tubing.

3. The method according to claim 1 wherein the intense, coherent light is obtained from a laser light source.

4. The method according to claim 1 wherein the wavelength of light is within the red spectrum.

5. The method according to claim 1 wherein the light has a wavelength within the range of 600-800 nanometers.

6. The method according to claim 5 wherein the wavelength of the light is approximately 638.2 nanometers.

7. The method according to claim 1 wherein the hematocrit being measured is known to be within a range of 15 units of hematocrit.

8. The method according to claim 7 wherein the hematocrit being measured is known to be within 30 and 45.

9. The method according to claim 7 wherein the hematocrit being measured is known to be within 35 and 50.

10. The method according to claim 7 wherein the hematocrit is known to be within 57 and 72.

11. The method according to claim 1 wherein said curve function is a linear function and said approximated curve fit is a linear approximation.

12. An apparatus for making a non-invasive, non-destructive, rapid, relatively accurate measurement in vivo of the hematocrit of undiluted blood including:

liquid confining means having a light transmitting portion and being adapted to receive undiluted blood therein;

means for generating an intense, coherent, monochromatic light beam and for directing said light beam through said light transmitting portion of said liquid confining means and the undiluted blood;

light sensing means arranged to detect the transmittance of light that passes through said light transmitting portion and through the undiluted blood from said light generating means and to produce an output signal related to the amount of transmitted light sensed and indicative of the light absorbed by the red blood cells; and

means for multiplying the output signal generated by the transmitted light sensed by a proportionality factor determined from a curve function obtained from an approximated curve fit of absorbance v. hematocrit measurements for measurements of light absorbance by blood having known hematocrits thereby to produce a corrected output signal which is indicative of the hematocrit of the undiluted blood in said liquid confining means and which can be used to control the flow rate of at least one pump pumping whole blood or a fluid component thereof in a blood processing apparatus.

13. The apparatus according to claim 12 wherein said light generating means is operable to generate light having a wavelength falling within the red spectrum.

14. The apparatus according to claim 12 wherein said light generating means is operable to generate light having a wavelength within the range of 600-800 nanometers.

15. The apparatus according to claim 12 wherein said light generating means includes a laser light source.

16. The apparatus according to claim 15 wherein said laser light source is an 0.5 milliwatt, neon-helium laser which produces light at a wavelength of approximately 638.2. nanometers.

17. The apparatus according to claim 12 wherein said liquid confining means is a tubing having a light transmitting portion.

18. The apparatus according to claim 17 wherein said tubing is transparent.

19. The apparatus according to claim 12 wherein said light generating and directing means includes a laser light source arranged to direct a laser light beam along a first path, a mirror positioned in said first path for reflecting the laser light beam along a second path 90°

from said first path and through said light transmitting portion of said liquid confining means, a second mirror arranged in said second path to receive transmitted light which has passed through said liquid confining means and to reflect that transmitted light along a third path 90°

from said second path and to said light sensing means.

20. The apparatus according to claim 13 wherein said light sensing means comprises a photodetector including a silicon diode and an amplifier circuit.

21. The apparatus according to claim 12 wherein said curve function is a linear function and said approximated curve fit is a linear approximation.

22. The apparatus according to claim 12 wherein said light sensing means includes an interference filter which is positioned to receive transmitted light and which passes only light of the wavelength of light generated by said light generating means.