Method and device for the semiquantitative determination of glucose in aqueous fluids
First Claim
1. In combination with the method of preparing a test device for the semiquantitative determination of glucose in urine which comprises impregnating a sheet of a bibulous carrier with a solution containing glucose oxidase, a substance having peroxidative activity and a water soluble iodide salt, drying the treated carrier and then saturating the carrier with an organic solution of a film-forming material, drying the carrier to leave a dry residue of the film-forming material and cutting the sheet into test strips, the improvement which comprises the use of a solution containing from about 1.75 to about 4.55 g./100 ml. of ethyl cellulose in a material which will solubilize the ethyl cellulose and will evaporate to leave a uniform residual layer of ethyl cellulose as solvent for the organic solution of film-forming material.
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Abstract
Disclosed is an improved method for the preparation of test strips for the semiquantitative determination of glucose in aqueous fluids, e.g. urine. The test strips are comprised of a bibulous carrier impregnated with glucose oxidase, a substance having peroxidative activity and a water soluble iodide salt. The device is coated with the residue of a solution of a film forming material which, pursuant to the improvement disclosed herein, is obtained by saturating the strip with a solution containing about 1.75 to about 4.55 grams (g)/100 milliliters (ml) of ethyl cellulose in an appropriate solvent.
37 Citations
15 Claims
- 1. In combination with the method of preparing a test device for the semiquantitative determination of glucose in urine which comprises impregnating a sheet of a bibulous carrier with a solution containing glucose oxidase, a substance having peroxidative activity and a water soluble iodide salt, drying the treated carrier and then saturating the carrier with an organic solution of a film-forming material, drying the carrier to leave a dry residue of the film-forming material and cutting the sheet into test strips, the improvement which comprises the use of a solution containing from about 1.75 to about 4.55 g./100 ml. of ethyl cellulose in a material which will solubilize the ethyl cellulose and will evaporate to leave a uniform residual layer of ethyl cellulose as solvent for the organic solution of film-forming material.
- 6. In combination with the method for the semiquantitative determination of glucose in urine which method comprises contacting the urine with a test device comprising a bibulous carrier impregnated with the residue of a solution containing glucose oxidase, a substance having peroxidative activity and water soluble iodide salt which device is coated with a hydrophobic, semi-permeable layer and determining the concentration of glucose in the urine from the color change of the device the improvement therein in which the layer is the dry residue created by saturating the device with a solution containing from about 1.75 to about 4.55 g./100 ml. of ethyl cellulose in a material which will solubilize the ethyl cellulose and will evaporate to leave a uniform residual layer of ethyl cellulose as solvent for the ethyl cellulose and removing the solvent.
- 11. In a test device for determining the presence of glucose in a liquid test sample, wherein said device comprises a carrier matrix impregnated with glucose oxidase, a peroxidatively active substance and a water soluble iodide salt, the improvement wherein said carrier matrix is additionally impregnated with ethyl cellulose by saturating said carrier with an organic solution containing about 1.75 to about 4.55 grams of ethyl cellulose per 100 milliliters of solvent.
Specification