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Immobilized enzyme membrane

  • US 4,307,195 A
  • Filed: 09/28/1979
  • Issued: 12/22/1981
  • Est. Priority Date: 09/29/1978
  • Status: Expired due to Term
First Claim
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1. An enzyme electrode apparatus for electrochemically analyzing a substance in a sample solution, which comprises a vessel for encasing an electrolyte solution therein;

  • an anode;

    a cathode;

    an immobilized enzyme membrane containing a skin layer that functions as a barrier provided at a working face of the anode and/or cathode; and

    an electrolyte solution in the vessel;

    the immobilized enzyme membrane being provided by an asymmetrical membrane having a thickness of 30-300 μ

    m integrally formed from a skin layer substantially incapable of permeating an enzyme therethrough, but capable of permeating a gas and a liquid therethrough, and a sponge layer having sufficient porosity for retaining a necessary amount of the enzyme and pores intercommunicated with one another throughout the sponge layer, the enzyme being immobilized in the pores of the sponge layer by cross-linking, the skin layer having a thickness of 0.1-3 m m and the sponge layer having a porosity of 50-90%;

    the skin layer and the sponge layer being formed from the same material.

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