Immobilized enzyme membrane

• US 4,307,195 A
• Filed: 09/28/1979
• Issued: 12/22/1981
• Est. Priority Date: 09/29/1978
• Status: Expired due to Term

• Current Assignee: Hitachi, Ltd.
• Original Assignee: Hitachi, Ltd.
• Inventors: Karasawa, Yoshiharu, Kohkame, Hisashi
• Primary Examiner(s): Naff, David M.
• Application Number:
  US06/080,026
• Time in Patent Office: 816 Days
• Field of Search: 435/174, 435/180, 435/181, 435/177, 435/182, 435/179, 435/288, 210/22, 204/1 T
• US Class Current: 204/403.1
• CPC Class Codes: B01D 69/144 containing embedded or bound biomolecules
• CPC Class Codes: C12N 11/02 Enzymes or microbial cells immobilised on or in an organic carrier
• CPC Class Codes: C12Q 1/002 Electrode membranes
• CPC Class Codes: G01N 27/3271 Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood amperometry per se G01N27/49; aspects concerning the enzyme reagent C12Q1/001

Abstract

An immobilized enzyme membrane for use at the working face of an electrochemical electrode is prepared which comprises an asymmetrical membrane integrally formed from a skin layer substantially incapable of permeating an enzyme therethrough but capable of permeating a gas and a liquid, and a sponge layer having pores containing an enzyme immobilized therein by crosslinking and which pores intercommunicate with one another throughout the sponge layer and provide sufficient porosity for retaining a necessary amount of the enzyme. The immobilized enzyme membrane contains a large amount of enzyme, has good diffusion and permeability, and has stabilized enzyme activity for a prolonged period of time. Additionally, there is obtained a quick response time and good analytical precision when the immobilized enzyme membrane is used at the working face of an electrode of electrochemical measuring instruments.

Claims

1. An enzyme electrode apparatus for electrochemically analyzing a substance in a sample solution, which comprises a vessel for encasing an electrolyte solution therein;

an anode;

a cathode;

an immobilized enzyme membrane containing a skin layer that functions as a barrier provided at a working face of the anode and/or cathode; and

an electrolyte solution in the vessel;

the immobilized enzyme membrane being provided by an asymmetrical membrane having a thickness of 30-300 μ

m integrally formed from a skin layer substantially incapable of permeating an enzyme therethrough, but capable of permeating a gas and a liquid therethrough, and a sponge layer having sufficient porosity for retaining a necessary amount of the enzyme and pores intercommunicated with one another throughout the sponge layer, the enzyme being immobilized in the pores of the sponge layer by cross-linking, the skin layer having a thickness of 0.1-3 m m and the sponge layer having a porosity of 50-90%;

the skin layer and the sponge layer being formed from the same material.

2. An enzyme electrode apparatus according to claim 1, wherein the asymmetrical membrane is made from a material selected from the group consisting of cellulose derivatives, polyamide and polyamino acid.

3. An enzyme electrode apparatus according to claim 1, wherein the immobilized enzyme membrane is provided at a working face of the anode.

4. An enzyme electrode apparatus according to claim 1 wherein the immobilized enzyme membrane is provided at a working face of the anode and a working face of the cathode.

5. An enzyme electrode apparatus according to claim 1, wherein the immobilized enzyme membrane is provided at a working face of the cathode.

6. An enzyme electrode apparatus according to claim 1, wherein the sponge layer has pores at the surface of the sponge layer with pore sizes of about 100 to about 500 nm.

7. An enzyme electrode apparatus according to claim 1, wherein the enzyme immobilized within said pores of the sponge layer by cross-linking is selected from the group consisting of glucose oxidase, amino acid oxidase, cholesterol oxidase, uricase, urease, creatininase, glutaminase, penicillinase, catalase, peroxidase, invertase, mutanotase, amylase, protease, and glucose isomerase.