Spectrophotometer

• US 4,310,249 A
• Filed: 10/09/1979
• Issued: 01/12/1982
• Est. Priority Date: 10/09/1979
• Status: Expired due to Term

• Current Assignee: Miles Laboratories, Inc. (Bayer AG)
• Original Assignee: Miles Laboratories, Inc. (Bayer AG)
• Inventors: Kramer, Donald L.
• Primary Examiner(s): Evans, F. L.
• Application Number:
  US06/082,674
• Time in Patent Office: 826 Days
• Field of Search: 356/414, 356/236, 356/73, 356/432, 356/436, 356/440, 250/228
• US Class Current: 356/414
• CPC Class Codes: G01N 21/255 Details, e.g. use of specially adapted sources, lighting or optical systems

Abstract

Apparatus for measuring transmitted light is described. The apparatus comprises a spectrophotometer which includes a light source; first and second diffusion chambers; a cuvette chamber and photodetection means.

The apparatus also includes light transmitting channels through which light can pass from said source to the first diffusion chamber, from the first diffusion chamber through the cuvette chamber to the second diffusion chamber, and from the second diffusion chamber to the photodetector means.

In the preferred embodiment the first and second diffusion chambers are light diffusing integrating spheres, and a filter is present in the light transmission channel between the light source and first diffusion chamber.

Claims

1. Spectrophotometer apparatus comprising:

a discontinuous light source capable of producing a light characteristic of at least 5,000°

K for a duration of from about one microsecond to about 100 microseconds;

a first light integrating sphere capable of uniformly diffusing light from said light source;

means defining a first light transmission path for the passage of light from said light source to said first light integrating sphere;

a second light integrating sphere capable of uniformly diffusing light;

a cylindrical liquid sample cuvette positioned between said first and second light integrating spheres;

means defining a second light transmission path for the passage of light from said first light integrating sphere through said sample cuvette to said second light integrating sphere, said second light transmission path being aligned at an angle to the first light transmission path;

photodetection means; and

means defining a third light transmission path for the passage of diffused light directly from said second light integrating sphere to said photodetection means, said third light transmission path being aligned at an angle to the second light transmission path.

2. Spectrophotometer of claim 1 which also includes a filter positioned in said first light transmission path, said filter permitting the transmission of light having a wavelength of between about 250 and about 800 nanometers.

3. Spectrophotometer apparatus of claim 1 in which the third light transmission path is aligned at a right angle to the second light transmission path.