Mobile phase supplying method in the liquid chromatography and apparatus therefor

• US 4,310,420 A
• Filed: 03/04/1980
• Issued: 01/12/1982
• Est. Priority Date: 03/14/1979
• Status: Expired due to Term

• Current Assignee: Japan Spectroscopic Company Limited
• Original Assignee: Japan Spectroscopic Company Limited
• Inventors: Konishi, Hideki, Sugawara, Michiaki, Saito, Muneo
• Primary Examiner(s): Adee, John
• Application Number:
  US06/127,994
• Time in Patent Office: 679 Days
• Field of Search: 210/659, 210/198.2, 210/101, 210/138, 210/745
• US Class Current: 210/659
• CPC Class Codes: B01D 15/14 relating to the introduction of the feed to the apparatus
• CPC Class Codes: B01D 15/166 Fluid composition conditioning, e.g. gradient
• CPC Class Codes: F04B 13/02 of two or more fluids at the same time
• CPC Class Codes: F04B 23/06 the pumps being all of reciprocating positive-displacement type
• CPC Class Codes: F04B 49/20 by changing the driving speed
• CPC Class Codes: G01N 2030/326 pumps
• CPC Class Codes: G01N 30/32 of pressure or speed G01N30/36 takes precedence

Abstract

A mobile phase supplying method in a liquid chromatography which is characterized in a disposition of a second plunger pump unit, in addition to the conventional way of employing one plunger pump unit (a first pump). The second plunger pump unit is disposed in series with the first plunger pump unit, being connected to the suction side of the first pump with the discharge side of the second pump, in order to synchronously supply the same amount of mobile phase discharged from the first pump to the suction side thereof by the second pump for the purpose of preventing the suction side of the first pump from being exposed to an occurrence of negative pressure. The apparatus for realizing the method is provided, in its double-pumped structure, with a connecting rod having on either end thereof a first and second plungers, between both pump units, which are synchronously and coaxially driven by a common driving system.

Claims

1. A method of supplying the mobile phase to a liquid chromatograph wherein said mobile phase consists of a predetermined mixture of a plurality of solvents each contained in separate reservoir means;

providing valve means and controlling the flow of each solvent out of its reservoir means using said value means;

providing a first reciprocation type plunger pump to supply said mobile phase and a sample therein to a liquid chromatograph;

providing a second reciprocation type plunger pump to supply said mobile phase to the suction side of said first pump;

synchronously driving both said pumps to secure a supply of said mobile phase to the first pump by supplying the same amount thereof discharged by said second pump;

controlling said valve means such that all of said solvents are prevented from flowing into said second pump while said second pump is carrying out its discharging operation; and

controlling the second pump when it is carrying out its sucking operations of the solvents from each reservoir via said valve means according to a predetermined time ratio or according to a predetermined flow amounts ratio,

whereby said predetermined mixture of said plurality of solvents in said mobile phase comprises predetermined amounts of and predetermined kinds of said solvents to be sucked into said second pump.

2. The method in accordance with claim 1, wherein the discharge amount of said mobile phase in one reciprocation of said second pump is equal to the suction amount of said mobile phase in one reciprocation of said first pump.

3. The method in accordance with claim 2, wherein both pumps are driven such that the time duration of the discharging operation by said second pump is equal to the time duration of the sucking operation by said first pump.

4. The method in accordance with claim 2, wherein the ratio;

T_s1 /T_d1 between the time duration;

T_s1 required for the sucking operation and the time duration;

T_d1 required for the discharging operation of said first pump is one or less.

5. The method in accordance with claim 1, 2, 3 or 4, wherein the ratio;

T_s2 /T_d2 between the time duration;

T_s2 required for the sucking operation and the time duration;

T_d2 required for the discharging operation of said second pump is one or more.

6. In a liquid chromatograph of the type wherein a mobile phase which consists of a predetermined mixture of a plurality of solvents is supplied to liquid chromatographic separation means for separating a sample introduced into the mobile phase, a mobile phase supplying apparatus comprising:

a first plunger pump unit and means to connect the discharge side thereof to said separation means;

a second plunger pump unit of substantially the same capacity of discharge and suction as that of said first plunger pump unit,

a plurality of reservoirs, one for each of said plurality of solvents;

valve means to control the flow of solvents out of said reservoirs;

means to control said valve means such that all of said solvents are prevented from flowing into said second pump while said second pump is carrying out its discharging operation;

the discharge side of said second pump unit being connected to the suction side of said first pump unit;

an axially slidable connecting rod between both of said plunger pump units, means to axially attach the ends of said rod to the plunger of each of said pump units, respectively;

a driving system for synchronously and reciprocally driving both plungers via said connecting rod to cause said first pump unit and said second pump unit to carry out reversed actions, sucking and discharging, respectively; and

control means for said valve means to cause the time duration of the second pump carrying out its sucking operations of the solvents from each reservior to be according to a predetermined time ratio or according to a predetermined flow amount ratio, whereby said predetermined mixture in said mobile phase comprises predetermined amounts of and predetermined kinds of said solvents to be sucked into said second pump.

7. The apparatus in accordance with claim 6, wherein said driving system is composed of a cam follower mounted on said connecting rod, a cam abutted on said cam follower for urging said connecting rod axial by, and means for rotating said cam at a predetermined speed.

8. The combination of claim 6, wherein said control means comprises an optical encoder associated with the driving system for said connecting rod.