Polysaccharides containing allose
First Claim
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1. A polysaccharide containing allose as a constituent sugar, said polysaccharide having the following identifying characteristics:
- (a) a molecular weight ranging from about 1×
104 to about 1×
107 ;
(b) no clear melting point;
(c) a content of from about 6 to about 12 mole % allose, from about 50 to about 60 mole % galactose, from about 10 to about 20 mole % glucose, from about 7 to about 14 mole % mannose and from 10 to about 12 mole % glucuronic acid;
(d) infra-red spectra showing O-H stretching in the vicinity of 3,400 cm-1, C--H stretching in the vicinity of 2,890 cm-1 and stretching of carboxylate anions in the vicinity of 1,620 cm-1 due to the orientation of beta-glucosido linkages in the vicinity of 800 cm-1 ;
(e) no distinct characteristic ultraviolet absorption spectra;
(f) solubility in water but insolubility in methanol, ethanol, ethers and acetone;
(g) the following color reactions;
anthrone reaction--positiveninhydrin reaction--negativeDische'"'"'s carbazole reaction--positiveDische'"'"'s cysteine-sulfuric acid reaction--positive;
(h) a specific rotation in water [α
]D25 of from about +30°
C.;
(i) forms a colorless, transparent and viscous solution with water; and
,(j) is aggregated to a gel in an aqueous solution containing calcium ion.
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Abstract
A new polysaccharide including allose as a constituent sugar and further characterized by galactose as a major constituent sugar is described. The polysaccharide is produced extracellularly by cultivation of Pseudomonas viscogena strains in nutrient medium.
45 Citations
6 Claims
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1. A polysaccharide containing allose as a constituent sugar, said polysaccharide having the following identifying characteristics:
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(a) a molecular weight ranging from about 1×
104 to about 1×
107 ;(b) no clear melting point; (c) a content of from about 6 to about 12 mole % allose, from about 50 to about 60 mole % galactose, from about 10 to about 20 mole % glucose, from about 7 to about 14 mole % mannose and from 10 to about 12 mole % glucuronic acid; (d) infra-red spectra showing O-H stretching in the vicinity of 3,400 cm-1, C--H stretching in the vicinity of 2,890 cm-1 and stretching of carboxylate anions in the vicinity of 1,620 cm-1 due to the orientation of beta-glucosido linkages in the vicinity of 800 cm-1 ; (e) no distinct characteristic ultraviolet absorption spectra; (f) solubility in water but insolubility in methanol, ethanol, ethers and acetone; (g) the following color reactions; anthrone reaction--positive ninhydrin reaction--negative Dische'"'"'s carbazole reaction--positive Dische'"'"'s cysteine-sulfuric acid reaction--positive; (h) a specific rotation in water [α
]D25 of from about +30°
C.;(i) forms a colorless, transparent and viscous solution with water; and
,(j) is aggregated to a gel in an aqueous solution containing calcium ion. - View Dependent Claims (2, 3, 4, 5, 6)
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Current AssigneeToyo Soda Manufacturing Co., Ltd.
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Original AssigneeToyo Soda Manufacturing Co., Ltd.
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InventorsIgarashi, Tatsuo, Takemoto, Hisao
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Primary Examiner(s)BROWN, JOHNNIE
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Application NumberUS06/030,444Time in Patent Office1,016 DaysField of Search536/1, 536/18, 536/114, 536/4, 435/101US Class Current536/123CPC Class CodesC08B 37/00 Preparation of polysacchari...C12P 19/04 Polysaccharides, i.e. compo...
